| DataBase | ID | URL or Descriptions |
|---|---|---|
| # ALTERNATIVE PRODUCTS | DPO3X_ECOLI | Event=Ribosomal frameshifting; Named isoforms=2; Comment=The production of the two protein products from this region is due to programmed ribosomal frameshifting. Frameshifting is about 40% efficient. {ECO 0000269|PubMed 2181440, ECO 0000269|PubMed 2186364, ECO 0000269|PubMed 2187190}; Name=tau; IsoId=P06710-1; Sequence=Displayed; Note=Produced by full-length translation of the dnaX gene.; Name=gamma; IsoId=P06710-2; Sequence=VSP_042848, VSP_042849; Note=Formed by programmed ribosomal frameshifting to a premature stop codon in the -1 frame at codon 430, the last residue is thus Glu and not Ser.; |
| # AltName | DPO3X_ECOLI | DNA polymerase III subunit gamma |
| # BioGrid | 4259846 | 114 |
| # CATALYTIC ACTIVITY | DPO3X_ECOLI | Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). |
| # EcoGene | EG10245 | dnaX |
| # FUNCTION | DPO3X_ECOLI | DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. |
| # FUNCTION | DPO3X_ECOLI | Isoform gamma seems to interact with the delta subunit. to transfer the beta subunit on the DNA. |
| # FUNCTION | DPO3X_ECOLI | Isoform tau serves as a scaffold to help in the dimerization of the core complex. |
| # GO_component | GO:0043846 | DNA polymerase III, clamp loader complex; IDA:EcoliWiki. |
| # GO_function | GO:0003677 | DNA binding; IEA:InterPro. |
| # GO_function | GO:0003887 | DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. |
| # GO_function | GO:0005524 | ATP binding; IEA:UniProtKB-KW. |
| # GO_function | GO:0016887 | ATPase activity; IDA:EcoliWiki. |
| # GO_function | GO:0017111 | nucleoside-triphosphatase activity; IDA:EcoliWiki. |
| # GO_function | GO:0030337 | DNA polymerase processivity factor activity; IDA:EcoliWiki. |
| # GO_function | GO:0042802 | identical protein binding; IPI:IntAct. |
| # GO_process | GO:0006260 | DNA replication; IMP:EcoliWiki. |
| # GO_process | GO:0006261 | DNA-dependent DNA replication; IBA:GO_Central. |
| # GOslim_component | GO:0043234 | protein complex |
| # GOslim_function | GO:0003674 | molecular_function |
| # GOslim_function | GO:0003677 | DNA binding |
| # GOslim_function | GO:0016779 | nucleotidyltransferase activity |
| # GOslim_function | GO:0016887 | ATPase activity |
| # GOslim_function | GO:0030234 | enzyme regulator activity |
| # GOslim_function | GO:0043167 | ion binding |
| # GOslim_process | GO:0006259 | DNA metabolic process |
| # GOslim_process | GO:0009058 | biosynthetic process |
| # Gene3D | 3.40.50.300 | -; 1. |
| # INTERACTION | DPO3X_ECOLI | Self; NbExp=5; IntAct=EBI-549140, EBI-549140; P11989 bglG; NbExp=3; IntAct=EBI-549140, EBI-545674; P0ACB0 dnaB; NbExp=2; IntAct=EBI-549140, EBI-548978; P10443 dnaE; NbExp=10; IntAct=EBI-549140, EBI-549111; P0A988 dnaN; NbExp=4; IntAct=EBI-549140, EBI-542385; P03007 dnaQ; NbExp=4; IntAct=EBI-549140, EBI-549131; P20605 fic; NbExp=2; IntAct=EBI-549140, EBI-1132602; P28630 holA; NbExp=6; IntAct=EBI-2604194, EBI-549153; P28631 holB; NbExp=17; IntAct=EBI-549140, EBI-549161; P28905 holC; NbExp=13; IntAct=EBI-549140, EBI-549169; P28632 holD; NbExp=18; IntAct=EBI-549140, EBI-549176; P23367 mutL; NbExp=2; IntAct=EBI-2604194, EBI-554913; P0AGE0 ssb; NbExp=2; IntAct=EBI-549140, EBI-1118620; |
| # IntAct | P06710 | 33 |
| # InterPro | IPR001270 | ClpA/B |
| # InterPro | IPR003593 | AAA+_ATPase |
| # InterPro | IPR008921 | DNA_pol3_clamp-load_cplx_C |
| # InterPro | IPR012763 | DNA_pol_III_sug/sutau |
| # InterPro | IPR021029 | DNA_pol_III_tau_dom-5 |
| # InterPro | IPR022001 | DNA_pol3_tau_IV |
| # InterPro | IPR022754 | DNA_pol_III_gamma-3 |
| # InterPro | IPR027417 | P-loop_NTPase |
| # KEGG_Brite | ko00001 | KEGG Orthology (KO) |
| # KEGG_Brite | ko00002 | KEGG pathway modules |
| # KEGG_Brite | ko01000 | Enzymes |
| # KEGG_Brite | ko03032 | DNA replication proteins |
| # KEGG_Brite | ko03032 | M00260 DNA polymerase III complex, bacteria |
| # KEGG_Brite | ko03400 | DNA repair and recombination proteins |
| # KEGG_Brite | ko03400 | M00260 DNA polymerase III complex, bacteria |
| # KEGG_Pathway | ko00230 | Purine metabolism |
| # KEGG_Pathway | ko00240 | Pyrimidine metabolism |
| # KEGG_Pathway | ko03030 | DNA replication |
| # KEGG_Pathway | ko03430 | Mismatch repair |
| # KEGG_Pathway | ko03440 | Homologous recombination |
| # Organism | DPO3X_ECOLI | Escherichia coli (strain K12) |
| # PATRIC | 32116097 | VBIEscCol129921_0490 |
| # PDB | 1JR3 | X-ray; 2.70 A; A/B/C=1-373 |
| # PDB | 1NJF | X-ray; 2.30 A; A/B/C/D=1-243 |
| # PDB | 1NJG | X-ray; 2.20 A; A/B=1-243 |
| # PDB | 1XXH | X-ray; 3.45 A; B/C/D/G/H/I=1-373 |
| # PDB | 1XXI | X-ray; 4.10 A; B/C/D/G/H/I=1-368 |
| # PDB | 2AYA | NMR; -; A=499-625 |
| # PDB | 3GLF | X-ray; 3.39 A; B/C/D/G/H/I=1-373 |
| # PDB | 3GLG | X-ray; 3.25 A; B/C/D/G/H/I=1-373 |
| # PDB | 3GLH | X-ray; 3.89 A; B/C/D/G/H/I/L/M/N=1-373 |
| # PDB | 3GLI | X-ray; 3.50 A; B/C/D/G/H/I=1-373 |
| # PDB | 5FKU | EM; 8.34 A; E=500-643 |
| # PDB | 5FKV | EM; 8.00 A; E=500-643 |
| # PIR | A25549 | DJEC3G |
| # PRINTS | PR00300 | CLPPROTEASEA |
| # Pfam | PF12168 | DNA_pol3_tau_4 |
| # Pfam | PF12169 | DNA_pol3_gamma3 |
| # Pfam | PF12170 | DNA_pol3_tau_5 |
| # Proteomes | UP000000318 | Chromosome |
| # Proteomes | UP000000625 | Chromosome |
| # RecName | DPO3X_ECOLI | DNA polymerase III subunit tau |
| # RefSeq | NP_415003 | NC_000913.3 |
| # RefSeq | WP_000122013 | NZ_LN832404.1 |
| # SEQUENCE CAUTION | Sequence=CAA28175.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO | 0000305}; |
| # SIMILARITY | Belongs to the DnaX/STICHEL family. {ECO | 0000305}. |
| # SMART | SM00382 | AAA |
| # SUBUNIT | The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha, epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, isoform gamma and tau. The composition of the holoenzyme is, therefore | (alpha,epsilon,theta)[2]-(isoform:gamma/tau)[3]-delta,delta', psi,chi-beta[4]. |
| # SUPFAM | SSF48019 | SSF48019 |
| # SUPFAM | SSF52540 | SSF52540 |
| # TIGRFAMs | TIGR02397 | dnaX_nterm |
| # eggNOG | COG2812 | LUCA |
| # eggNOG | ENOG4107QMP | Bacteria |
| BLAST | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/kblast/swissprot:DPO3X_ECOLI |
| BioCyc | ECOL316407:JW0459-MONOMER | http://biocyc.org/getid?id=ECOL316407:JW0459-MONOMER |
| BioCyc | EcoCyc:EG10245-MONOMER | http://biocyc.org/getid?id=EcoCyc:EG10245-MONOMER |
| BioCyc | EcoCyc:MONOMER0-2383 | http://biocyc.org/getid?id=EcoCyc:MONOMER0-2383 |
| BioCyc | MetaCyc:EG10245-MONOMER | http://biocyc.org/getid?id=MetaCyc:EG10245-MONOMER |
| BioCyc | MetaCyc:MONOMER0-2383 | http://biocyc.org/getid?id=MetaCyc:MONOMER0-2383 |
| COG | COG2812 | http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2812 |
| DIP | DIP-9464N | http://dip.doe-mbi.ucla.edu/dip/Browse.cgi?ID=DIP-9464N |
| DOI | 10.1002/bies.950140206 | http://dx.doi.org/10.1002/bies.950140206 |
| DOI | 10.1002/elps.1150180805 | http://dx.doi.org/10.1002/elps.1150180805 |
| DOI | 10.1038/msb4100049 | http://dx.doi.org/10.1038/msb4100049 |
| DOI | 10.1073/pnas.87.10.3713 | http://dx.doi.org/10.1073/pnas.87.10.3713 |
| DOI | 10.1073/pnas.87.7.2516 | http://dx.doi.org/10.1073/pnas.87.7.2516 |
| DOI | 10.1093/nar/14.16.6541 | http://dx.doi.org/10.1093/nar/14.16.6541 |
| DOI | 10.1093/nar/14.20.8091 | http://dx.doi.org/10.1093/nar/14.20.8091 |
| DOI | 10.1093/nar/18.7.1725 | http://dx.doi.org/10.1093/nar/18.7.1725 |
| DOI | 10.1126/science.277.5331.1453 | http://dx.doi.org/10.1126/science.277.5331.1453 |
| EC_number | EC:2.7.7.7 | http://www.genome.jp/dbget-bin/www_bget?EC:2.7.7.7 |
| EMBL | AP009048 | http://www.ebi.ac.uk/ena/data/view/AP009048 |
| EMBL | M38777 | http://www.ebi.ac.uk/ena/data/view/M38777 |
| EMBL | U00096 | http://www.ebi.ac.uk/ena/data/view/U00096 |
| EMBL | U82664 | http://www.ebi.ac.uk/ena/data/view/U82664 |
| EMBL | X04275 | http://www.ebi.ac.uk/ena/data/view/X04275 |
| EMBL | X04487 | http://www.ebi.ac.uk/ena/data/view/X04487 |
| EMBL | X04487 | http://www.ebi.ac.uk/ena/data/view/X04487 |
| ENZYME | 2.7.7.7 | http://enzyme.expasy.org/EC/2.7.7.7 |
| EchoBASE | EB0241 | http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0241 |
| EcoGene | EG10245 | http://www.ecogene.org/geneInfo.php?eg_id=EG10245 |
| EnsemblBacteria | AAC73572 | http://www.ensemblgenomes.org/id/AAC73572 |
| EnsemblBacteria | AAC73572 | http://www.ensemblgenomes.org/id/AAC73572 |
| EnsemblBacteria | BAE76249 | http://www.ensemblgenomes.org/id/BAE76249 |
| EnsemblBacteria | BAE76249 | http://www.ensemblgenomes.org/id/BAE76249 |
| EnsemblBacteria | BAE76249 | http://www.ensemblgenomes.org/id/BAE76249 |
| EnsemblBacteria | b0470 | http://www.ensemblgenomes.org/id/b0470 |
| G-Links | NC_000913 | http://link.g-language.org/NC_000913/format=tsv |
| GO_component | GO:0043846 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043846 |
| GO_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GO_function | GO:0003887 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003887 |
| GO_function | GO:0005524 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005524 |
| GO_function | GO:0016887 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016887 |
| GO_function | GO:0017111 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0017111 |
| GO_function | GO:0030337 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0030337 |
| GO_function | GO:0042802 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0042802 |
| GO_process | GO:0006260 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006260 |
| GO_process | GO:0006261 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006261 |
| GOslim_component | GO:0043234 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043234 |
| GOslim_function | GO:0003674 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003674 |
| GOslim_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GOslim_function | GO:0016779 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016779 |
| GOslim_function | GO:0016887 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016887 |
| GOslim_function | GO:0030234 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0030234 |
| GOslim_function | GO:0043167 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043167 |
| GOslim_process | GO:0006259 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006259 |
| GOslim_process | GO:0009058 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009058 |
| Gene3D | 3.40.50.300 | http://www.cathdb.info/version/latest/superfamily/3.40.50.300 |
| GeneID | 945105 | http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=945105 |
| HOGENOM | HOG000083934 | http://pbil.univ-lyon1.fr/cgi-bin/view-tree.pl?query=HOG000083934&db=HOGENOM6 |
| InParanoid | P06710 | http://inparanoid.sbc.su.se/cgi-bin/gene_search.cgi?id=P06710 |
| IntAct | P06710 | http://www.ebi.ac.uk/intact/pages/interactions/interactions.xhtml?query=P06710* |
| IntEnz | 2.7.7.7 | http://www.ebi.ac.uk/intenz/query?cmd=Search&q=2.7.7.7 |
| InterPro | IPR001270 | http://www.ebi.ac.uk/interpro/entry/IPR001270 |
| InterPro | IPR003593 | http://www.ebi.ac.uk/interpro/entry/IPR003593 |
| InterPro | IPR008921 | http://www.ebi.ac.uk/interpro/entry/IPR008921 |
| InterPro | IPR012763 | http://www.ebi.ac.uk/interpro/entry/IPR012763 |
| InterPro | IPR021029 | http://www.ebi.ac.uk/interpro/entry/IPR021029 |
| InterPro | IPR022001 | http://www.ebi.ac.uk/interpro/entry/IPR022001 |
| InterPro | IPR022754 | http://www.ebi.ac.uk/interpro/entry/IPR022754 |
| InterPro | IPR027417 | http://www.ebi.ac.uk/interpro/entry/IPR027417 |
| KEGG_Brite | ko00001 | http://www.genome.jp/dbget-bin/www_bget?ko00001 |
| KEGG_Brite | ko00002 | http://www.genome.jp/dbget-bin/www_bget?ko00002 |
| KEGG_Brite | ko01000 | http://www.genome.jp/dbget-bin/www_bget?ko01000 |
| KEGG_Brite | ko03032 | http://www.genome.jp/dbget-bin/www_bget?ko03032 |
| KEGG_Brite | ko03032 | http://www.genome.jp/dbget-bin/www_bget?ko03032 |
| KEGG_Brite | ko03400 | http://www.genome.jp/dbget-bin/www_bget?ko03400 |
| KEGG_Brite | ko03400 | http://www.genome.jp/dbget-bin/www_bget?ko03400 |
| KEGG_Gene | ecj:JW0459 | http://www.genome.jp/dbget-bin/www_bget?ecj:JW0459 |
| KEGG_Gene | eco:b0470 | http://www.genome.jp/dbget-bin/www_bget?eco:b0470 |
| KEGG_Orthology | KO:K02343 | http://www.genome.jp/dbget-bin/www_bget?KO:K02343 |
| KEGG_Pathway | ko00230 | http://www.genome.jp/kegg-bin/show_pathway?ko00230 |
| KEGG_Pathway | ko00240 | http://www.genome.jp/kegg-bin/show_pathway?ko00240 |
| KEGG_Pathway | ko03030 | http://www.genome.jp/kegg-bin/show_pathway?ko03030 |
| KEGG_Pathway | ko03430 | http://www.genome.jp/kegg-bin/show_pathway?ko03430 |
| KEGG_Pathway | ko03440 | http://www.genome.jp/kegg-bin/show_pathway?ko03440 |
| KEGG_Reaction | rn:R00375 | http://www.genome.jp/dbget-bin/www_bget?rn:R00375 |
| KEGG_Reaction | rn:R00376 | http://www.genome.jp/dbget-bin/www_bget?rn:R00376 |
| KEGG_Reaction | rn:R00377 | http://www.genome.jp/dbget-bin/www_bget?rn:R00377 |
| KEGG_Reaction | rn:R00378 | http://www.genome.jp/dbget-bin/www_bget?rn:R00378 |
| MINT | MINT-1222776 | http://mint.bio.uniroma2.it/mint/search/search.do?queryType=protein&interactorAc=MINT-1222776 |
| OMA | TALEHEK | http://omabrowser.org/cgi-bin/gateway.pl?f=DisplayGroup&p1=TALEHEK |
| PDB | 1JR3 | http://www.ebi.ac.uk/pdbe-srv/view/entry/1JR3 |
| PDB | 1NJF | http://www.ebi.ac.uk/pdbe-srv/view/entry/1NJF |
| PDB | 1NJG | http://www.ebi.ac.uk/pdbe-srv/view/entry/1NJG |
| PDB | 1XXH | http://www.ebi.ac.uk/pdbe-srv/view/entry/1XXH |
| PDB | 1XXI | http://www.ebi.ac.uk/pdbe-srv/view/entry/1XXI |
| PDB | 2AYA | http://www.ebi.ac.uk/pdbe-srv/view/entry/2AYA |
| PDB | 3GLF | http://www.ebi.ac.uk/pdbe-srv/view/entry/3GLF |
| PDB | 3GLG | http://www.ebi.ac.uk/pdbe-srv/view/entry/3GLG |
| PDB | 3GLH | http://www.ebi.ac.uk/pdbe-srv/view/entry/3GLH |
| PDB | 3GLI | http://www.ebi.ac.uk/pdbe-srv/view/entry/3GLI |
| PDB | 5FKU | http://www.ebi.ac.uk/pdbe-srv/view/entry/5FKU |
| PDB | 5FKV | http://www.ebi.ac.uk/pdbe-srv/view/entry/5FKV |
| PDBsum | 1JR3 | http://www.ebi.ac.uk/pdbsum/1JR3 |
| PDBsum | 1NJF | http://www.ebi.ac.uk/pdbsum/1NJF |
| PDBsum | 1NJG | http://www.ebi.ac.uk/pdbsum/1NJG |
| PDBsum | 1XXH | http://www.ebi.ac.uk/pdbsum/1XXH |
| PDBsum | 1XXI | http://www.ebi.ac.uk/pdbsum/1XXI |
| PDBsum | 2AYA | http://www.ebi.ac.uk/pdbsum/2AYA |
| PDBsum | 3GLF | http://www.ebi.ac.uk/pdbsum/3GLF |
| PDBsum | 3GLG | http://www.ebi.ac.uk/pdbsum/3GLG |
| PDBsum | 3GLH | http://www.ebi.ac.uk/pdbsum/3GLH |
| PDBsum | 3GLI | http://www.ebi.ac.uk/pdbsum/3GLI |
| PDBsum | 5FKU | http://www.ebi.ac.uk/pdbsum/5FKU |
| PDBsum | 5FKV | http://www.ebi.ac.uk/pdbsum/5FKV |
| PRINTS | PR00300 | http://umber.sbs.man.ac.uk/cgi-bin/dbbrowser/sprint/searchprintss.cgi?display_opts=Prints&category=None&queryform=false&prints_accn=PR00300 |
| PSORT-B | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/kpsortb/swissprot:DPO3X_ECOLI |
| PSORT2 | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/kpsort2/swissprot:DPO3X_ECOLI |
| PSORT | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/kpsort/swissprot:DPO3X_ECOLI |
| Pfam | PF12168 | http://pfam.xfam.org/family/PF12168 |
| Pfam | PF12169 | http://pfam.xfam.org/family/PF12169 |
| Pfam | PF12170 | http://pfam.xfam.org/family/PF12170 |
| Phobius | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/kphobius/swissprot:DPO3X_ECOLI |
| PhylomeDB | P06710 | http://phylomedb.org/?seqid=P06710 |
| ProteinModelPortal | P06710 | http://www.proteinmodelportal.org/query/uniprot/P06710 |
| PubMed | 1575709 | http://www.ncbi.nlm.nih.gov/pubmed/1575709 |
| PubMed | 16738553 | http://www.ncbi.nlm.nih.gov/pubmed/16738553 |
| PubMed | 2181440 | http://www.ncbi.nlm.nih.gov/pubmed/2181440 |
| PubMed | 2186364 | http://www.ncbi.nlm.nih.gov/pubmed/2186364 |
| PubMed | 2187190 | http://www.ncbi.nlm.nih.gov/pubmed/2187190 |
| PubMed | 3018672 | http://www.ncbi.nlm.nih.gov/pubmed/3018672 |
| PubMed | 3283125 | http://www.ncbi.nlm.nih.gov/pubmed/3283125 |
| PubMed | 3534795 | http://www.ncbi.nlm.nih.gov/pubmed/3534795 |
| PubMed | 9278503 | http://www.ncbi.nlm.nih.gov/pubmed/9278503 |
| PubMed | 9298644 | http://www.ncbi.nlm.nih.gov/pubmed/9298644 |
| RefSeq | NP_415003 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=NP_415003 |
| RefSeq | WP_000122013 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=WP_000122013 |
| SMART | SM00382 | http://smart.embl.de/smart/do_annotation.pl?DOMAIN=SM00382 |
| SMR | P06710 | http://swissmodel.expasy.org/repository/smr.php?sptr_ac=P06710 |
| STRING | 511145.b0470 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=511145.b0470&targetmode=cogs |
| STRING | COG2812 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=COG2812&targetmode=cogs |
| SUPFAM | SSF48019 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF48019 |
| SUPFAM | SSF52540 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF52540 |
| TIGRFAMs | TIGR02397 | http://cmr.jcvi.org/tigr-scripts/CMR/HmmReport.cgi?hmm_acc=TIGR02397 |
| UniProtKB-AC | P06710 | http://www.uniprot.org/uniprot/P06710 |
| UniProtKB | DPO3X_ECOLI | http://www.uniprot.org/uniprot/DPO3X_ECOLI |
| charge | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/charge/swissprot:DPO3X_ECOLI |
| eggNOG | COG2812 | http://eggnogapi.embl.de/nog_data/html/tree/COG2812 |
| eggNOG | ENOG4107QMP | http://eggnogapi.embl.de/nog_data/html/tree/ENOG4107QMP |
| epestfind | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/epestfind/swissprot:DPO3X_ECOLI |
| garnier | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/garnier/swissprot:DPO3X_ECOLI |
| helixturnhelix | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/helixturnhelix/swissprot:DPO3X_ECOLI |
| hmoment | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/hmoment/swissprot:DPO3X_ECOLI |
| iep | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/iep/swissprot:DPO3X_ECOLI |
| inforesidue | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/inforesidue/swissprot:DPO3X_ECOLI |
| octanol | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/octanol/swissprot:DPO3X_ECOLI |
| pepcoil | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepcoil/swissprot:DPO3X_ECOLI |
| pepdigest | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepdigest/swissprot:DPO3X_ECOLI |
| pepinfo | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepinfo/swissprot:DPO3X_ECOLI |
| pepnet | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepnet/swissprot:DPO3X_ECOLI |
| pepstats | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepstats/swissprot:DPO3X_ECOLI |
| pepwheel | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepwheel/swissprot:DPO3X_ECOLI |
| pepwindow | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/pepwindow/swissprot:DPO3X_ECOLI |
| sigcleave | swissprot:DPO3X_ECOLI | http://rest.g-language.org/emboss/sigcleave/swissprot:DPO3X_ECOLI |
| DataBase | ID | URL or Descriptions |
|---|---|---|
| # AltName | HDA_ECOLI | DnaA paralog |
| # BioGrid | 4261436 | 149 |
| # DISRUPTION PHENOTYPE | HDA_ECOLI | Essential in strain C600. Disruption in MG1655 confers cold-sensitivity with asynchronously replicating DNA, which is quickly suppressed. Increased levels of plasmid IncP-alpha RK2 in strain BL21-DE3, increased plasmid replication in vitro. {ECO 0000269|PubMed 11483528, ECO 0000269|PubMed 12618445, ECO 0000269|PubMed 22716942}. |
| # EcoGene | EG14201 | hda |
| # FUNCTION | HDA_ECOLI | Mediates the interactions of DNA replication initiator protein DnaA with DNA polymerase subunit beta sliding clamp (dnaN). Stimulates hydrolysis of ATP-DnaA to ADP-DnaA, rendering DnaA inactive for reinitiation, a process called regulatory inhibition of DnaA or RIDA. ADP-binding activates Hda to hydrolyze DnaA-ATP; Hda monomers bind to ADP with about 200-fold greater affinity than for ATP. RIDA function can be genetically separated from viability, suggesting this protein has another function as well. |
| # FUNCTION | HDA_ECOLI | Suppresses the toxic effect of overexpressing a TrfA N- terminal 163 residue fragment. Inhibits inner membrane-associated plasmid IncP-alpha RK2 replication probably by interacting with plasmid-encoded TrfA. |
| # GO_component | GO:0005829 | cytosol; IDA:EcoCyc. |
| # GO_component | GO:0005886 | plasma membrane; IEA:UniProtKB-SubCell. |
| # GO_component | GO:0016020 | membrane; IDA:EcoCyc. |
| # GO_function | GO:0000166 | nucleotide binding; IEA:UniProtKB-KW. |
| # GO_function | GO:0043565 | sequence-specific DNA binding; IBA:GO_Central. |
| # GO_process | GO:0006260 | DNA replication; IEA:UniProtKB-HAMAP. |
| # GO_process | GO:0032297 | negative regulation of DNA-dependent DNA replication initiation; IMP:EcoCyc. |
| # GOslim_component | GO:0005575 | cellular_component |
| # GOslim_component | GO:0005829 | cytosol |
| # GOslim_component | GO:0005886 | plasma membrane |
| # GOslim_function | GO:0003674 | molecular_function |
| # GOslim_function | GO:0003677 | DNA binding |
| # GOslim_process | GO:0006259 | DNA metabolic process |
| # GOslim_process | GO:0008150 | biological_process |
| # GOslim_process | GO:0009058 | biosynthetic process |
| # Gene3D | 3.40.50.300 | -; 1. |
| # HAMAP | MF_01158 | Hda |
| # INTERACTION | HDA_ECOLI | Self; NbExp=2; IntAct=EBI-545453, EBI-545453; P06134 ada; NbExp=3; IntAct=EBI-545453, EBI-1119501; P03004 dnaA; NbExp=2; IntAct=EBI-545453, EBI-548951; P0A988 dnaN; NbExp=8; IntAct=EBI-545453, EBI-542385; |
| # IntAct | P69931 | 14 |
| # InterPro | IPR013317 | DnaA |
| # InterPro | IPR017788 | Hda |
| # InterPro | IPR020591 | Chromosome_initiator_DnaA-like |
| # InterPro | IPR022864 | Hda_Enterobact |
| # InterPro | IPR027417 | P-loop_NTPase |
| # KEGG_Brite | ko03032 | DNA replication proteins |
| # MISCELLANEOUS | HDA_ECOLI | Starts with a CTG codon. |
| # MISCELLANEOUS | HDA_ECOLI | There are about 50 homodimers per cell in strains C600 and K12 / MG1655. |
| # Organism | HDA_ECOLI | Escherichia coli (strain K12) |
| # PATRIC | 32120379 | VBIEscCol129921_2592 |
| # PIR | G65025 | G65025 |
| # PRINTS | PR00051 | DNAA |
| # Pfam | PF00308 | Bac_DnaA |
| # Proteomes | UP000000318 | Chromosome |
| # Proteomes | UP000000625 | Chromosome |
| # RecName | HDA_ECOLI | DnaA regulatory inactivator Hda |
| # RefSeq | NP_416991 | NC_000913.3 |
| # SIMILARITY | Belongs to the DnaA family. HdA subfamily. {ECO | 0000305}. |
| # SUBCELLULAR LOCATION | HDA_ECOLI | Cell inner membrane {ECO 0000269|PubMed 12618445}. Note=More protein is found in the inner than outer membrane fractions. |
| # SUBUNIT | HDA_ECOLI | The active form seems to be an ADP-bound monomer; apo-Hda forms homo-multimers that do not hydrolzye DnaA-bound ATP. Forms the RIDA complex (regulatory inactivation of DnaA) of ATP-DnaA, ADP-Hda and the DNA-loaded beta sliding clamp (dnaN). Interacts with plasmid IncP-alpha RK2-encoded protein TrfA in strain B / BL21-DE3. {ECO 0000269|PubMed 12618445, ECO 0000269|PubMed 15150238, ECO 0000269|PubMed 15611053, ECO 0000269|PubMed 18977760}. |
| # SUPFAM | SSF52540 | SSF52540 |
| # TIGRFAMs | TIGR03420 | DnaA_homol_Hda |
| # eggNOG | COG0593 | LUCA |
| # eggNOG | ENOG4108KZ1 | Bacteria |
| BLAST | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/kblast/swissprot:HDA_ECOLI |
| BioCyc | ECOL316407:JW5397-MONOMER | http://biocyc.org/getid?id=ECOL316407:JW5397-MONOMER |
| BioCyc | EcoCyc:G7313-MONOMER | http://biocyc.org/getid?id=EcoCyc:G7313-MONOMER |
| COG | COG0593 | http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0593 |
| DIP | DIP-48006N | http://dip.doe-mbi.ucla.edu/dip/Browse.cgi?ID=DIP-48006N |
| DOI | 10.1038/msb4100049 | http://dx.doi.org/10.1038/msb4100049 |
| DOI | 10.1074/jbc.M412060200 | http://dx.doi.org/10.1074/jbc.M412060200 |
| DOI | 10.1074/jbc.M803158200 | http://dx.doi.org/10.1074/jbc.M803158200 |
| DOI | 10.1093/dnares/4.2.91 | http://dx.doi.org/10.1093/dnares/4.2.91 |
| DOI | 10.1093/emboj/20.15.4253 | http://dx.doi.org/10.1093/emboj/20.15.4253 |
| DOI | 10.1111/j.1365-2958.2012.08129.x | http://dx.doi.org/10.1111/j.1365-2958.2012.08129.x |
| DOI | 10.1126/science.277.5331.1453 | http://dx.doi.org/10.1126/science.277.5331.1453 |
| DOI | 10.1128/JB.185.10.3244-3248.2003 | http://dx.doi.org/10.1128/JB.185.10.3244-3248.2003 |
| DOI | 10.1128/JB.185.6.1817-1824.2003 | http://dx.doi.org/10.1128/JB.185.6.1817-1824.2003 |
| DOI | 10.1128/JB.186.11.3508-3515.2004 | http://dx.doi.org/10.1128/JB.186.11.3508-3515.2004 |
| EMBL | AP009048 | http://www.ebi.ac.uk/ena/data/view/AP009048 |
| EMBL | U00096 | http://www.ebi.ac.uk/ena/data/view/U00096 |
| EchoBASE | EB3953 | http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3953 |
| EcoGene | EG14201 | http://www.ecogene.org/geneInfo.php?eg_id=EG14201 |
| EnsemblBacteria | AAC75549 | http://www.ensemblgenomes.org/id/AAC75549 |
| EnsemblBacteria | AAC75549 | http://www.ensemblgenomes.org/id/AAC75549 |
| EnsemblBacteria | BAA16384 | http://www.ensemblgenomes.org/id/BAA16384 |
| EnsemblBacteria | BAA16384 | http://www.ensemblgenomes.org/id/BAA16384 |
| EnsemblBacteria | BAA16384 | http://www.ensemblgenomes.org/id/BAA16384 |
| EnsemblBacteria | b2496 | http://www.ensemblgenomes.org/id/b2496 |
| G-Links | NC_000913 | http://link.g-language.org/NC_000913/format=tsv |
| GO_component | GO:0005829 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005829 |
| GO_component | GO:0005886 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005886 |
| GO_component | GO:0016020 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016020 |
| GO_function | GO:0000166 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0000166 |
| GO_function | GO:0043565 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043565 |
| GO_process | GO:0006260 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006260 |
| GO_process | GO:0032297 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0032297 |
| GOslim_component | GO:0005575 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005575 |
| GOslim_component | GO:0005829 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005829 |
| GOslim_component | GO:0005886 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005886 |
| GOslim_function | GO:0003674 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003674 |
| GOslim_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GOslim_process | GO:0006259 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006259 |
| GOslim_process | GO:0008150 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0008150 |
| GOslim_process | GO:0009058 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009058 |
| Gene3D | 3.40.50.300 | http://www.cathdb.info/version/latest/superfamily/3.40.50.300 |
| GeneID | 946977 | http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=946977 |
| HAMAP | MF_01158 | http://hamap.expasy.org/unirule/MF_01158 |
| HOGENOM | HOG000256538 | http://pbil.univ-lyon1.fr/cgi-bin/view-tree.pl?query=HOG000256538&db=HOGENOM6 |
| InParanoid | P69931 | http://inparanoid.sbc.su.se/cgi-bin/gene_search.cgi?id=P69931 |
| IntAct | P69931 | http://www.ebi.ac.uk/intact/pages/interactions/interactions.xhtml?query=P69931* |
| InterPro | IPR013317 | http://www.ebi.ac.uk/interpro/entry/IPR013317 |
| InterPro | IPR017788 | http://www.ebi.ac.uk/interpro/entry/IPR017788 |
| InterPro | IPR020591 | http://www.ebi.ac.uk/interpro/entry/IPR020591 |
| InterPro | IPR022864 | http://www.ebi.ac.uk/interpro/entry/IPR022864 |
| InterPro | IPR027417 | http://www.ebi.ac.uk/interpro/entry/IPR027417 |
| KEGG_Brite | ko03032 | http://www.genome.jp/dbget-bin/www_bget?ko03032 |
| KEGG_Gene | ecj:JW5397 | http://www.genome.jp/dbget-bin/www_bget?ecj:JW5397 |
| KEGG_Gene | eco:b2496 | http://www.genome.jp/dbget-bin/www_bget?eco:b2496 |
| KEGG_Orthology | KO:K10763 | http://www.genome.jp/dbget-bin/www_bget?KO:K10763 |
| PRINTS | PR00051 | http://umber.sbs.man.ac.uk/cgi-bin/dbbrowser/sprint/searchprintss.cgi?display_opts=Prints&category=None&queryform=false&prints_accn=PR00051 |
| PSORT-B | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/kpsortb/swissprot:HDA_ECOLI |
| PSORT2 | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/kpsort2/swissprot:HDA_ECOLI |
| PSORT | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/kpsort/swissprot:HDA_ECOLI |
| Pfam | PF00308 | http://pfam.xfam.org/family/PF00308 |
| Phobius | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/kphobius/swissprot:HDA_ECOLI |
| PhylomeDB | P69931 | http://phylomedb.org/?seqid=P69931 |
| ProteinModelPortal | P69931 | http://www.proteinmodelportal.org/query/uniprot/P69931 |
| PubMed | 11483528 | http://www.ncbi.nlm.nih.gov/pubmed/11483528 |
| PubMed | 12618445 | http://www.ncbi.nlm.nih.gov/pubmed/12618445 |
| PubMed | 12730188 | http://www.ncbi.nlm.nih.gov/pubmed/12730188 |
| PubMed | 15150238 | http://www.ncbi.nlm.nih.gov/pubmed/15150238 |
| PubMed | 15611053 | http://www.ncbi.nlm.nih.gov/pubmed/15611053 |
| PubMed | 16738553 | http://www.ncbi.nlm.nih.gov/pubmed/16738553 |
| PubMed | 18977760 | http://www.ncbi.nlm.nih.gov/pubmed/18977760 |
| PubMed | 22716942 | http://www.ncbi.nlm.nih.gov/pubmed/22716942 |
| PubMed | 9205837 | http://www.ncbi.nlm.nih.gov/pubmed/9205837 |
| PubMed | 9278503 | http://www.ncbi.nlm.nih.gov/pubmed/9278503 |
| RefSeq | NP_416991 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=NP_416991 |
| SMR | P69931 | http://swissmodel.expasy.org/repository/smr.php?sptr_ac=P69931 |
| STRING | 511145.b2496 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=511145.b2496&targetmode=cogs |
| STRING | COG0593 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=COG0593&targetmode=cogs |
| SUPFAM | SSF52540 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF52540 |
| TIGRFAMs | TIGR03420 | http://cmr.jcvi.org/tigr-scripts/CMR/HmmReport.cgi?hmm_acc=TIGR03420 |
| UniProtKB-AC | P69931 | http://www.uniprot.org/uniprot/P69931 |
| UniProtKB | HDA_ECOLI | http://www.uniprot.org/uniprot/HDA_ECOLI |
| charge | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/charge/swissprot:HDA_ECOLI |
| eggNOG | COG0593 | http://eggnogapi.embl.de/nog_data/html/tree/COG0593 |
| eggNOG | ENOG4108KZ1 | http://eggnogapi.embl.de/nog_data/html/tree/ENOG4108KZ1 |
| epestfind | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/epestfind/swissprot:HDA_ECOLI |
| garnier | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/garnier/swissprot:HDA_ECOLI |
| helixturnhelix | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/helixturnhelix/swissprot:HDA_ECOLI |
| hmoment | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/hmoment/swissprot:HDA_ECOLI |
| iep | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/iep/swissprot:HDA_ECOLI |
| inforesidue | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/inforesidue/swissprot:HDA_ECOLI |
| octanol | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/octanol/swissprot:HDA_ECOLI |
| pepcoil | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepcoil/swissprot:HDA_ECOLI |
| pepdigest | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepdigest/swissprot:HDA_ECOLI |
| pepinfo | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepinfo/swissprot:HDA_ECOLI |
| pepnet | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepnet/swissprot:HDA_ECOLI |
| pepstats | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepstats/swissprot:HDA_ECOLI |
| pepwheel | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepwheel/swissprot:HDA_ECOLI |
| pepwindow | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/pepwindow/swissprot:HDA_ECOLI |
| sigcleave | swissprot:HDA_ECOLI | http://rest.g-language.org/emboss/sigcleave/swissprot:HDA_ECOLI |
| DataBase | ID | URL or Descriptions |
|---|---|---|
| # AltName | GYRB_ECOLI | Type IIA topoisomerase subunit GyrB |
| # BRENDA | 5.99.1 | 2026 |
| # BioGrid | 4259537 | 154 |
| # CATALYTIC ACTIVITY | GYRB_ECOLI | ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO 0000255|HAMAP-Rule MF_01898, ECO 0000269|PubMed 12051842, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 18642932, ECO 0000269|PubMed 186775, ECO 0000269|PubMed 19965760, ECO 0000269|PubMed 20675723, ECO 0000269|PubMed 22731783}. |
| # COFACTOR | GYRB_ECOLI | Name=K(+); Xref=ChEBI CHEBI 29103; Evidence={ECO 0000269|PubMed 25849408}; Note=Binds one K(+) per subunit which interacts with the alpha- phosphate of ATP analog and stimulates ATPase activity of the N- terminal fragment; Na(+) or water bind less well (PubMed 25849408). {ECO 0000269|PubMed 25849408}; |
| # COFACTOR | GYRB_ECOLI | Name=Mg(2+); Xref=ChEBI CHEBI 18420; Evidence={ECO 0000255|HAMAP-Rule MF_01898, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 18642932}; Name=Mn(2+); Xref=ChEBI CHEBI 29035; Evidence={ECO 0000255|HAMAP-Rule MF_01898, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 18642932}; Name=Ca(2+); Xref=ChEBI CHEBI 29108; Evidence={ECO 0000255|HAMAP-Rule MF_01898, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 18642932}; Note=Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+) (PubMed 12051843, PubMed 18642932). {ECO 0000255|HAMAP-Rule MF_01898, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 18642932}; |
| # COFACTOR | GYRB_ECOLI | Name=Na(+); Xref=ChEBI CHEBI 29101; Evidence={ECO 0000269|PubMed 25849408}; Note=Binds one Na(+) per subunit, with 4 ligands provided by water; may be able to bind K(+), the functional significance of this ion is unclear (PubMed 25849408). {ECO 0000269|PubMed 25849408}; |
| # DOMAIN | GYRB_ECOLI | Consists of 3 domains; the ATPase domain (residues 1-220), the transducer domain (221-392) and the toprim domain (393-804) (PubMed 1646964, PubMed 10734094). ATP-binding is cooperative, and both subunits must be wild-type at residue 103 for supercoiling to occur (PubMed 8621650). Non-hydrolyzable ATP analogs (and ATP- binding) induce dimerization and enhance ATPase activity (PubMed 10734094, PubMed 9657678). ATP hydrolysis induces domain shifts that are probably part of the mechanism of DNA cleavage and rejoining (PubMed 25202966). {ECO 0000269|PubMed 10734094, ECO 0000269|PubMed 1646964, ECO 0000269|PubMed 8621650, ECO 0000305|PubMed 25202966, ECO 0000305|PubMed 9657678}. |
| # DrugBank | DB00817 | Rosoxacin |
| # ENZYME REGULATION | GYRB_ECOLI | Gyrase is the target of many classes of inhibitors, including coumarins, cyclothialidines, pyrrolopyrimidines, pyrazolthiazoles and (fluoro)quinolones. Coumarins bind to GyrB and are competitive inhibitors of its ATPase activity (PubMed 7811004). Cyclothialidines also bind GyrB and are ATPase competitive inhibitors; they seem to act differently from coumarins (PubMed 7811004, PubMed 8635474). Pyrrolopyrimidines inhibit both GyrB and its paralog in topoisomerase 4 (parE) (PubMed 23294697, PubMed 23352267, PubMed 24386374). Pyrazolthiazoles also inhibit the ATPase activity of GyrB (PubMed 20356737). Quinolones bind GyrA when the enzyme is complexed with DNA and trap the enzyme in a covalent reaction intermediate with DNA (PubMed 3031051, PubMed 12051842, PubMed 337300). Acriflavine inhibits DNA supercoiling and DNA- stimulated ATPase activity (PubMed 9148951). DNA supercoiling activity is protected from fluoroquinolone inhibition by QnrB4; QnrB4 has no effect on supercoiling activity alone (PubMed 19060136). {ECO 0000269|PubMed 19060136, ECO 0000269|PubMed 20356737, ECO 0000269|PubMed 23294697, ECO 0000269|PubMed 23352267, ECO 0000269|PubMed 24386374, ECO 0000269|PubMed 3031051, ECO 0000269|PubMed 337300, ECO 0000269|PubMed 7811004, ECO 0000269|PubMed 8635474, ECO 0000269|PubMed 9148951, ECO 0000305|PubMed 12051842}. |
| # EcoGene | EG10424 | gyrB |
| # FUNCTION | GYRB_ECOLI | DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner to maintain chromosomes in an underwound state (PubMed 186775, PubMed 3031051, PubMed 1323022, PubMed 8248233, PubMed 7811004, PubMed 8621650, PubMed 9657678, PubMed 12051842, PubMed 12051843, PubMed 18642932, PubMed 19060136, PubMed 19965760, PubMed 22457353, PubMed 23294697, PubMed 20356737, PubMed 20675723, PubMed 23352267, PubMed 24386374, PubMed 25202966, PubMed 25849408). This makes better substrates for topoisomerase 4 (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli (PubMed 9334322). Gyrase catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes (PubMed 22457352). Relaxes negatively supercoiled DNA in an ATP-independent manner (PubMed 337300). E.coli gyrase has higher supercoiling activity than other characterized bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli (PubMed 22457352). E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical (PubMed 17400739). The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C- terminal domain (approximately residues 524-841) and specifically the GyrA-box (PubMed 8962066, PubMed 16332690). {ECO 0000269|PubMed 12051842, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 1323022, ECO 0000269|PubMed 16332690, ECO 0000269|PubMed 17400739, ECO 0000269|PubMed 18642932, ECO 0000269|PubMed 186775, ECO 0000269|PubMed 19060136, ECO 0000269|PubMed 19965760, ECO 0000269|PubMed 20356737, ECO 0000269|PubMed 20675723, ECO 0000269|PubMed 22457352, ECO 0000269|PubMed 22457353, ECO 0000269|PubMed 23294697, ECO 0000269|PubMed 23352267, ECO 0000269|PubMed 24386374, ECO 0000269|PubMed 25202966, ECO 0000269|PubMed 25849408, ECO 0000269|PubMed 3031051, ECO 0000269|PubMed 337300, ECO 0000269|PubMed 7811004, ECO 0000269|PubMed 8248233, ECO 0000269|PubMed 8621650, ECO 0000269|PubMed 8962066, ECO 0000269|PubMed 9148951, ECO 0000269|PubMed 9334322, ECO 0000269|PubMed 9657678}. |
| # GO_component | GO:0005694 | chromosome; IEA:InterPro. |
| # GO_component | GO:0005737 | cytoplasm; IDA:EcoliWiki. |
| # GO_component | GO:0005829 | cytosol; IDA:EcoCyc. |
| # GO_component | GO:0009295 | nucleoid; IBA:GO_Central. |
| # GO_component | GO:0009330 | DNA topoisomerase complex (ATP-hydrolyzing); IDA:EcoliWiki. |
| # GO_function | GO:0000287 | magnesium ion binding; IEA:UniProtKB-HAMAP. |
| # GO_function | GO:0003677 | DNA binding; IDA:EcoliWiki. |
| # GO_function | GO:0003918 | DNA topoisomerase type II (ATP-hydrolyzing) activity; IDA:EcoliWiki. |
| # GO_function | GO:0005524 | ATP binding; IDA:EcoliWiki. |
| # GO_function | GO:0008094 | DNA-dependent ATPase activity; IDA:EcoliWiki. |
| # GO_function | GO:0034335 | DNA supercoiling activity; IDA:UniProtKB. |
| # GO_process | GO:0006265 | DNA topological change; IMP:EcoliWiki. |
| # GO_process | GO:0006268 | DNA unwinding involved in DNA replication; IBA:GO_Central. |
| # GO_process | GO:0006351 | transcription, DNA-templated; IMP:EcoliWiki. |
| # GO_process | GO:0007059 | chromosome segregation; IBA:GO_Central. |
| # GO_process | GO:0042493 | response to drug; IDA:EcoliWiki. |
| # GO_process | GO:0046677 | response to antibiotic; IEA:UniProtKB-KW. |
| # GOslim_component | GO:0005575 | cellular_component |
| # GOslim_component | GO:0005694 | chromosome |
| # GOslim_component | GO:0005737 | cytoplasm |
| # GOslim_component | GO:0005829 | cytosol |
| # GOslim_component | GO:0043234 | protein complex |
| # GOslim_function | GO:0003677 | DNA binding |
| # GOslim_function | GO:0016853 | isomerase activity |
| # GOslim_function | GO:0016887 | ATPase activity |
| # GOslim_function | GO:0043167 | ion binding |
| # GOslim_process | GO:0006259 | DNA metabolic process |
| # GOslim_process | GO:0007059 | chromosome segregation |
| # GOslim_process | GO:0008150 | biological_process |
| # GOslim_process | GO:0009058 | biosynthetic process |
| # GOslim_process | GO:0034641 | cellular nitrogen compound metabolic process |
| # GOslim_process | GO:0051276 | chromosome organization |
| # Gene3D | 3.30.230.10 | -; 1. |
| # Gene3D | 3.30.565.10 | -; 1. |
| # Gene3D | 3.40.50.670 | -; 2. |
| # HAMAP | MF_01898 | GyrB |
| # INTERACTION | GYRB_ECOLI | P0AES4 gyrA; NbExp=5; IntAct=EBI-541911, EBI-547129; |
| # IntAct | P0AES6 | 18 |
| # InterPro | IPR001241 | Topo_IIA |
| # InterPro | IPR002288 | DNA_gyrase_B_C |
| # InterPro | IPR003594 | HATPase_C |
| # InterPro | IPR006171 | Toprim_domain |
| # InterPro | IPR011557 | GyrB |
| # InterPro | IPR013506 | Topo_IIA_bsu_dom2 |
| # InterPro | IPR013759 | Topo_IIA_cen_dom |
| # InterPro | IPR013760 | Topo_IIA-like_dom |
| # InterPro | IPR014721 | Ribosomal_S5_D2-typ_fold_subgr |
| # InterPro | IPR018522 | TopoIIA_CS |
| # InterPro | IPR020568 | Ribosomal_S5_D2-typ_fold |
| # KEGG_Brite | ko01000 | Enzymes |
| # KEGG_Brite | ko03032 | DNA replication proteins |
| # KEGG_Brite | ko03400 | DNA repair and recombination proteins |
| # MISCELLANEOUS | GYRB_ECOLI | When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between GyrA and DNA in an ATP- independent manner (PubMed 3031051). In the presence of quinolones this intermediate can be trapped and is used as an indicator of drug toxicity (PubMed 12051842). {ECO 0000269|PubMed 3031051, ECO 0000305|PubMed 12051842}. |
| # Organism | GYRB_ECOLI | Escherichia coli (strain K12) |
| # PATRIC | 32122895 | VBIEscCol129921_3823 |
| # PDB | 1AJ6 | X-ray; 2.30 A; A=2-220 |
| # PDB | 1EI1 | X-ray; 2.30 A; A/B=2-392 |
| # PDB | 1KZN | X-ray; 2.30 A; A=15-219 |
| # PDB | 3G7E | X-ray; 2.20 A; A=15-217 |
| # PDB | 3NUH | X-ray; 3.10 A; B=389-804 |
| # PDB | 4DUH | X-ray; 1.50 A; A/B=1-220 |
| # PDB | 4HYP | X-ray; 2.60 A; A/B/C/D=15-220 |
| # PDB | 4KFG | X-ray; 1.60 A; A/B=15-220 |
| # PDB | 4PRV | X-ray; 2.00 A; A=2-392 |
| # PDB | 4PRX | X-ray; 1.80 A; A=2-392 |
| # PDB | 4PU9 | X-ray; 2.40 A; A=2-392 |
| # PDB | 4WUB | X-ray; 1.75 A; A=2-393 |
| # PDB | 4WUC | X-ray; 1.90 A; A=2-393 |
| # PDB | 4WUD | X-ray; 1.95 A; A=2-393 |
| # PDB | 4XTJ | X-ray; 1.92 A; A=2-392 |
| # PDB | 4ZVI | X-ray; 2.20 A; A=16-392 |
| # PDB | 5L3J | X-ray; 2.83 A; A=15-392 |
| # PIR | D65172 | ISECTB |
| # PRINTS | PR00418 | TPI2FAMILY |
| # PROSITE | PS00177 | TOPOISOMERASE_II |
| # PROSITE | PS50880 | TOPRIM |
| # Pfam | PF00204 | DNA_gyraseB |
| # Pfam | PF00986 | DNA_gyraseB_C |
| # Pfam | PF01751 | Toprim |
| # Pfam | PF02518 | HATPase_c |
| # Proteomes | UP000000318 | Chromosome |
| # Proteomes | UP000000625 | Chromosome |
| # RecName | DNA gyrase subunit B {ECO:0000255|HAMAP-Rule | MF_01898} |
| # RefSeq | WP_000072067 | NZ_LN832404.1 |
| # RefSeq | YP_026241 | NC_000913.3 |
| # SIMILARITY | Belongs to the type II topoisomerase family. {ECO:0000255|HAMAP-Rule | MF_01898}. |
| # SIMILARITY | Contains 1 Toprim domain. {ECO:0000255|HAMAP- Rule | MF_01898}. |
| # SMART | SM00387 | HATPase_c |
| # SMART | SM00433 | TOP2c |
| # SUBCELLULAR LOCATION | GYRB_ECOLI | Cytoplasm {ECO 0000255|HAMAP-Rule MF_01898}. |
| # SUBUNIT | GYRB_ECOLI | Heterotetramer, composed of two GyrA and two GyrB chains (PubMed 9148951, PubMed 12051842). In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with the DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis (PubMed 12051843, PubMed 18642932, PubMed 20675723, PubMed 19965760). {ECO 0000255|HAMAP- Rule MF_01898, ECO 0000269|PubMed 12051842, ECO 0000269|PubMed 12051843, ECO 0000269|PubMed 18642932, ECO 0000269|PubMed 19965760, ECO 0000269|PubMed 20675723, ECO 0000269|PubMed 9148951}. |
| # SUPFAM | SSF54211 | SSF54211 |
| # SUPFAM | SSF55874 | SSF55874 |
| # SUPFAM | SSF56719 | SSF56719; 2 |
| # TIGRFAMs | TIGR01059 | gyrB |
| # eggNOG | COG0187 | LUCA |
| # eggNOG | ENOG4105C7D | Bacteria |
| BLAST | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/kblast/swissprot:GYRB_ECOLI |
| BioCyc | ECOL316407:JW5625-MONOMER | http://biocyc.org/getid?id=ECOL316407:JW5625-MONOMER |
| BioCyc | EcoCyc:EG10424-MONOMER | http://biocyc.org/getid?id=EcoCyc:EG10424-MONOMER |
| BioCyc | MetaCyc:EG10424-MONOMER | http://biocyc.org/getid?id=MetaCyc:EG10424-MONOMER |
| COG | COG0187 | http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0187 |
| DIP | DIP-48005N | http://dip.doe-mbi.ucla.edu/dip/Browse.cgi?ID=DIP-48005N |
| DOI | 10.1002/(SICI)1097-0134(199705)28:1<41::AID-PROT4>3.3.CO | 3.3.CO>http://dx.doi.org/10.1002/(SICI)1097-0134(199705)28:1<41::AID-PROT4>3.3.CO |
| DOI | 10.1002/elps.1150180805 | http://dx.doi.org/10.1002/elps.1150180805 |
| DOI | 10.1006/geno.1993.1230 | http://dx.doi.org/10.1006/geno.1993.1230 |
| DOI | 10.1007/BF00331012 | http://dx.doi.org/10.1007/BF00331012 |
| DOI | 10.1016/S0022-2836(02)00048-7 | http://dx.doi.org/10.1016/S0022-2836(02)00048-7 |
| DOI | 10.1016/S0022-2836(02)00049-9 | http://dx.doi.org/10.1016/S0022-2836(02)00049-9 |
| DOI | 10.1016/j.bmcl.2010.03.052 | http://dx.doi.org/10.1016/j.bmcl.2010.03.052 |
| DOI | 10.1016/j.bmcl.2012.11.032 | http://dx.doi.org/10.1016/j.bmcl.2012.11.032 |
| DOI | 10.1016/j.bmcl.2012.11.073 | http://dx.doi.org/10.1016/j.bmcl.2012.11.073 |
| DOI | 10.1021/bi0159837 | http://dx.doi.org/10.1021/bi0159837 |
| DOI | 10.1021/bi800480j | http://dx.doi.org/10.1021/bi800480j |
| DOI | 10.1021/bi970294+ | http://dx.doi.org/10.1021/bi970294+ |
| DOI | 10.1021/bi9801309 | http://dx.doi.org/10.1021/bi9801309 |
| DOI | 10.1021/jm300395d | http://dx.doi.org/10.1021/jm300395d |
| DOI | 10.1038/351624a0 | http://dx.doi.org/10.1038/351624a0 |
| DOI | 10.1038/msb4100049 | http://dx.doi.org/10.1038/msb4100049 |
| DOI | 10.1073/pnas.73.11.3872 | http://dx.doi.org/10.1073/pnas.73.11.3872 |
| DOI | 10.1073/pnas.74.11.4772 | http://dx.doi.org/10.1073/pnas.74.11.4772 |
| DOI | 10.1073/pnas.90.23.11232 | http://dx.doi.org/10.1073/pnas.90.23.11232 |
| DOI | 10.1073/pnas.93.25.14416 | http://dx.doi.org/10.1073/pnas.93.25.14416 |
| DOI | 10.1074/jbc.271.16.9723 | http://dx.doi.org/10.1074/jbc.271.16.9723 |
| DOI | 10.1074/jbc.272.20.13302 | http://dx.doi.org/10.1074/jbc.272.20.13302 |
| DOI | 10.1074/jbc.275.13.9468 | http://dx.doi.org/10.1074/jbc.275.13.9468 |
| DOI | 10.1074/jbc.M112.345678 | http://dx.doi.org/10.1074/jbc.M112.345678 |
| DOI | 10.1074/jbc.M112.345736 | http://dx.doi.org/10.1074/jbc.M112.345736 |
| DOI | 10.1074/jbc.M511160200 | http://dx.doi.org/10.1074/jbc.M511160200 |
| DOI | 10.1093/nar/12.16.6389 | http://dx.doi.org/10.1093/nar/12.16.6389 |
| DOI | 10.1093/nar/15.2.771 | http://dx.doi.org/10.1093/nar/15.2.771 |
| DOI | 10.1093/nar/gkj405 | http://dx.doi.org/10.1093/nar/gkj405 |
| DOI | 10.1093/nar/gkq665 | http://dx.doi.org/10.1093/nar/gkq665 |
| DOI | 10.1101/gad.11.19.2580 | http://dx.doi.org/10.1101/gad.11.19.2580 |
| DOI | 10.1107/S1399004715002916 | http://dx.doi.org/10.1107/S1399004715002916 |
| DOI | 10.1111/j.1365-2958.1992.tb00886.x | http://dx.doi.org/10.1111/j.1365-2958.1992.tb00886.x |
| DOI | 10.1126/science.1179123 | http://dx.doi.org/10.1126/science.1179123 |
| DOI | 10.1126/science.277.5331.1453 | http://dx.doi.org/10.1126/science.277.5331.1453 |
| DOI | 10.1128/AAC.35.8.1647 | http://dx.doi.org/10.1128/AAC.35.8.1647 |
| DOI | 10.1128/AAC.38.9.1966 | http://dx.doi.org/10.1128/AAC.38.9.1966 |
| DOI | 10.1128/JB.00083-07 | http://dx.doi.org/10.1128/JB.00083-07 |
| DOI | 10.1128/JB.01205-08 | http://dx.doi.org/10.1128/JB.01205-08 |
| DOI | 10.1371/journal.pone.0084409 | http://dx.doi.org/10.1371/journal.pone.0084409 |
| DOI | 10.1371/journal.pone.0107289 | http://dx.doi.org/10.1371/journal.pone.0107289 |
| DrugBank | DB00817 | http://www.drugbank.ca/drugs/DB00817 |
| EC_number | EC:5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760} | http://www.genome.jp/dbget-bin/www_bget?EC:5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760} |
| EMBL | AP009048 | http://www.ebi.ac.uk/ena/data/view/AP009048 |
| EMBL | D87842 | http://www.ebi.ac.uk/ena/data/view/D87842 |
| EMBL | L10328 | http://www.ebi.ac.uk/ena/data/view/L10328 |
| EMBL | M15548 | http://www.ebi.ac.uk/ena/data/view/M15548 |
| EMBL | U00096 | http://www.ebi.ac.uk/ena/data/view/U00096 |
| EMBL | X04341 | http://www.ebi.ac.uk/ena/data/view/X04341 |
| ENZYME | 5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760} | http://enzyme.expasy.org/EC/5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760} |
| EchoBASE | EB0419 | http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0419 |
| EcoGene | EG10424 | http://www.ecogene.org/geneInfo.php?eg_id=EG10424 |
| EnsemblBacteria | AAT48201 | http://www.ensemblgenomes.org/id/AAT48201 |
| EnsemblBacteria | AAT48201 | http://www.ensemblgenomes.org/id/AAT48201 |
| EnsemblBacteria | BAE77595 | http://www.ensemblgenomes.org/id/BAE77595 |
| EnsemblBacteria | BAE77595 | http://www.ensemblgenomes.org/id/BAE77595 |
| EnsemblBacteria | BAE77595 | http://www.ensemblgenomes.org/id/BAE77595 |
| EnsemblBacteria | b3699 | http://www.ensemblgenomes.org/id/b3699 |
| G-Links | NC_000913 | http://link.g-language.org/NC_000913/format=tsv |
| GO_component | GO:0005694 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005694 |
| GO_component | GO:0005737 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005737 |
| GO_component | GO:0005829 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005829 |
| GO_component | GO:0009295 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009295 |
| GO_component | GO:0009330 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009330 |
| GO_function | GO:0000287 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0000287 |
| GO_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GO_function | GO:0003918 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003918 |
| GO_function | GO:0005524 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005524 |
| GO_function | GO:0008094 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0008094 |
| GO_function | GO:0034335 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0034335 |
| GO_process | GO:0006265 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006265 |
| GO_process | GO:0006268 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006268 |
| GO_process | GO:0006351 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006351 |
| GO_process | GO:0007059 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0007059 |
| GO_process | GO:0042493 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0042493 |
| GO_process | GO:0046677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0046677 |
| GOslim_component | GO:0005575 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005575 |
| GOslim_component | GO:0005694 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005694 |
| GOslim_component | GO:0005737 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005737 |
| GOslim_component | GO:0005829 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005829 |
| GOslim_component | GO:0043234 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043234 |
| GOslim_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GOslim_function | GO:0016853 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016853 |
| GOslim_function | GO:0016887 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016887 |
| GOslim_function | GO:0043167 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043167 |
| GOslim_process | GO:0006259 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006259 |
| GOslim_process | GO:0007059 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0007059 |
| GOslim_process | GO:0008150 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0008150 |
| GOslim_process | GO:0009058 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009058 |
| GOslim_process | GO:0034641 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0034641 |
| GOslim_process | GO:0051276 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0051276 |
| Gene3D | 3.30.230.10 | http://www.cathdb.info/version/latest/superfamily/3.30.230.10 |
| Gene3D | 3.30.565.10 | http://www.cathdb.info/version/latest/superfamily/3.30.565.10 |
| Gene3D | 3.40.50.670 | http://www.cathdb.info/version/latest/superfamily/3.40.50.670 |
| GeneID | 948211 | http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=948211 |
| HAMAP | MF_01898 | http://hamap.expasy.org/unirule/MF_01898 |
| HOGENOM | HOG000075155 | http://pbil.univ-lyon1.fr/cgi-bin/view-tree.pl?query=HOG000075155&db=HOGENOM6 |
| InParanoid | P0AES6 | http://inparanoid.sbc.su.se/cgi-bin/gene_search.cgi?id=P0AES6 |
| IntAct | P0AES6 | http://www.ebi.ac.uk/intact/pages/interactions/interactions.xhtml?query=P0AES6* |
| IntEnz | 5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760} | http://www.ebi.ac.uk/intenz/query?cmd=Search&q=5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:12051843, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760} |
| InterPro | IPR001241 | http://www.ebi.ac.uk/interpro/entry/IPR001241 |
| InterPro | IPR002288 | http://www.ebi.ac.uk/interpro/entry/IPR002288 |
| InterPro | IPR003594 | http://www.ebi.ac.uk/interpro/entry/IPR003594 |
| InterPro | IPR006171 | http://www.ebi.ac.uk/interpro/entry/IPR006171 |
| InterPro | IPR011557 | http://www.ebi.ac.uk/interpro/entry/IPR011557 |
| InterPro | IPR013506 | http://www.ebi.ac.uk/interpro/entry/IPR013506 |
| InterPro | IPR013759 | http://www.ebi.ac.uk/interpro/entry/IPR013759 |
| InterPro | IPR013760 | http://www.ebi.ac.uk/interpro/entry/IPR013760 |
| InterPro | IPR014721 | http://www.ebi.ac.uk/interpro/entry/IPR014721 |
| InterPro | IPR018522 | http://www.ebi.ac.uk/interpro/entry/IPR018522 |
| InterPro | IPR020568 | http://www.ebi.ac.uk/interpro/entry/IPR020568 |
| KEGG_Brite | ko01000 | http://www.genome.jp/dbget-bin/www_bget?ko01000 |
| KEGG_Brite | ko03032 | http://www.genome.jp/dbget-bin/www_bget?ko03032 |
| KEGG_Brite | ko03400 | http://www.genome.jp/dbget-bin/www_bget?ko03400 |
| KEGG_Gene | ecj:JW5625 | http://www.genome.jp/dbget-bin/www_bget?ecj:JW5625 |
| KEGG_Gene | eco:b3699 | http://www.genome.jp/dbget-bin/www_bget?eco:b3699 |
| KEGG_Orthology | KO:K02470 | http://www.genome.jp/dbget-bin/www_bget?KO:K02470 |
| OMA | IKNMITA | http://omabrowser.org/cgi-bin/gateway.pl?f=DisplayGroup&p1=IKNMITA |
| PDB | 1AJ6 | http://www.ebi.ac.uk/pdbe-srv/view/entry/1AJ6 |
| PDB | 1EI1 | http://www.ebi.ac.uk/pdbe-srv/view/entry/1EI1 |
| PDB | 1KZN | http://www.ebi.ac.uk/pdbe-srv/view/entry/1KZN |
| PDB | 3G7E | http://www.ebi.ac.uk/pdbe-srv/view/entry/3G7E |
| PDB | 3NUH | http://www.ebi.ac.uk/pdbe-srv/view/entry/3NUH |
| PDB | 4DUH | http://www.ebi.ac.uk/pdbe-srv/view/entry/4DUH |
| PDB | 4HYP | http://www.ebi.ac.uk/pdbe-srv/view/entry/4HYP |
| PDB | 4KFG | http://www.ebi.ac.uk/pdbe-srv/view/entry/4KFG |
| PDB | 4PRV | http://www.ebi.ac.uk/pdbe-srv/view/entry/4PRV |
| PDB | 4PRX | http://www.ebi.ac.uk/pdbe-srv/view/entry/4PRX |
| PDB | 4PU9 | http://www.ebi.ac.uk/pdbe-srv/view/entry/4PU9 |
| PDB | 4WUB | http://www.ebi.ac.uk/pdbe-srv/view/entry/4WUB |
| PDB | 4WUC | http://www.ebi.ac.uk/pdbe-srv/view/entry/4WUC |
| PDB | 4WUD | http://www.ebi.ac.uk/pdbe-srv/view/entry/4WUD |
| PDB | 4XTJ | http://www.ebi.ac.uk/pdbe-srv/view/entry/4XTJ |
| PDB | 4ZVI | http://www.ebi.ac.uk/pdbe-srv/view/entry/4ZVI |
| PDB | 5L3J | http://www.ebi.ac.uk/pdbe-srv/view/entry/5L3J |
| PDBsum | 1AJ6 | http://www.ebi.ac.uk/pdbsum/1AJ6 |
| PDBsum | 1EI1 | http://www.ebi.ac.uk/pdbsum/1EI1 |
| PDBsum | 1KZN | http://www.ebi.ac.uk/pdbsum/1KZN |
| PDBsum | 3G7E | http://www.ebi.ac.uk/pdbsum/3G7E |
| PDBsum | 3NUH | http://www.ebi.ac.uk/pdbsum/3NUH |
| PDBsum | 4DUH | http://www.ebi.ac.uk/pdbsum/4DUH |
| PDBsum | 4HYP | http://www.ebi.ac.uk/pdbsum/4HYP |
| PDBsum | 4KFG | http://www.ebi.ac.uk/pdbsum/4KFG |
| PDBsum | 4PRV | http://www.ebi.ac.uk/pdbsum/4PRV |
| PDBsum | 4PRX | http://www.ebi.ac.uk/pdbsum/4PRX |
| PDBsum | 4PU9 | http://www.ebi.ac.uk/pdbsum/4PU9 |
| PDBsum | 4WUB | http://www.ebi.ac.uk/pdbsum/4WUB |
| PDBsum | 4WUC | http://www.ebi.ac.uk/pdbsum/4WUC |
| PDBsum | 4WUD | http://www.ebi.ac.uk/pdbsum/4WUD |
| PDBsum | 4XTJ | http://www.ebi.ac.uk/pdbsum/4XTJ |
| PDBsum | 4ZVI | http://www.ebi.ac.uk/pdbsum/4ZVI |
| PDBsum | 5L3J | http://www.ebi.ac.uk/pdbsum/5L3J |
| PRINTS | PR00418 | http://umber.sbs.man.ac.uk/cgi-bin/dbbrowser/sprint/searchprintss.cgi?display_opts=Prints&category=None&queryform=false&prints_accn=PR00418 |
| PROSITE | PS00177 | http://prosite.expasy.org/cgi-bin/prosite/nicedoc.pl?PS00177 |
| PROSITE | PS50880 | http://prosite.expasy.org/cgi-bin/prosite/nicedoc.pl?PS50880 |
| PSORT-B | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/kpsortb/swissprot:GYRB_ECOLI |
| PSORT2 | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/kpsort2/swissprot:GYRB_ECOLI |
| PSORT | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/kpsort/swissprot:GYRB_ECOLI |
| Pfam | PF00204 | http://pfam.xfam.org/family/PF00204 |
| Pfam | PF00986 | http://pfam.xfam.org/family/PF00986 |
| Pfam | PF01751 | http://pfam.xfam.org/family/PF01751 |
| Pfam | PF02518 | http://pfam.xfam.org/family/PF02518 |
| Phobius | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/kphobius/swissprot:GYRB_ECOLI |
| PhylomeDB | P0AES6 | http://phylomedb.org/?seqid=P0AES6 |
| ProteinModelPortal | P0AES6 | http://www.proteinmodelportal.org/query/uniprot/P0AES6 |
| PubMed | 10734094 | http://www.ncbi.nlm.nih.gov/pubmed/10734094 |
| PubMed | 12044152 | http://www.ncbi.nlm.nih.gov/pubmed/12044152 |
| PubMed | 12051842 | http://www.ncbi.nlm.nih.gov/pubmed/12051842 |
| PubMed | 12051843 | http://www.ncbi.nlm.nih.gov/pubmed/12051843 |
| PubMed | 1323022 | http://www.ncbi.nlm.nih.gov/pubmed/1323022 |
| PubMed | 16332690 | http://www.ncbi.nlm.nih.gov/pubmed/16332690 |
| PubMed | 16397293 | http://www.ncbi.nlm.nih.gov/pubmed/16397293 |
| PubMed | 1646964 | http://www.ncbi.nlm.nih.gov/pubmed/1646964 |
| PubMed | 1656869 | http://www.ncbi.nlm.nih.gov/pubmed/1656869 |
| PubMed | 16738553 | http://www.ncbi.nlm.nih.gov/pubmed/16738553 |
| PubMed | 17400739 | http://www.ncbi.nlm.nih.gov/pubmed/17400739 |
| PubMed | 1846808 | http://www.ncbi.nlm.nih.gov/pubmed/1846808 |
| PubMed | 18642932 | http://www.ncbi.nlm.nih.gov/pubmed/18642932 |
| PubMed | 186775 | http://www.ncbi.nlm.nih.gov/pubmed/186775 |
| PubMed | 19060136 | http://www.ncbi.nlm.nih.gov/pubmed/19060136 |
| PubMed | 19965760 | http://www.ncbi.nlm.nih.gov/pubmed/19965760 |
| PubMed | 20356737 | http://www.ncbi.nlm.nih.gov/pubmed/20356737 |
| PubMed | 20675723 | http://www.ncbi.nlm.nih.gov/pubmed/20675723 |
| PubMed | 2174443 | http://www.ncbi.nlm.nih.gov/pubmed/2174443 |
| PubMed | 22457352 | http://www.ncbi.nlm.nih.gov/pubmed/22457352 |
| PubMed | 22457353 | http://www.ncbi.nlm.nih.gov/pubmed/22457353 |
| PubMed | 22731783 | http://www.ncbi.nlm.nih.gov/pubmed/22731783 |
| PubMed | 23294697 | http://www.ncbi.nlm.nih.gov/pubmed/23294697 |
| PubMed | 23352267 | http://www.ncbi.nlm.nih.gov/pubmed/23352267 |
| PubMed | 24386374 | http://www.ncbi.nlm.nih.gov/pubmed/24386374 |
| PubMed | 25202966 | http://www.ncbi.nlm.nih.gov/pubmed/25202966 |
| PubMed | 25849408 | http://www.ncbi.nlm.nih.gov/pubmed/25849408 |
| PubMed | 3020376 | http://www.ncbi.nlm.nih.gov/pubmed/3020376 |
| PubMed | 3029031 | http://www.ncbi.nlm.nih.gov/pubmed/3029031 |
| PubMed | 3029692 | http://www.ncbi.nlm.nih.gov/pubmed/3029692 |
| PubMed | 3031051 | http://www.ncbi.nlm.nih.gov/pubmed/3031051 |
| PubMed | 337300 | http://www.ncbi.nlm.nih.gov/pubmed/337300 |
| PubMed | 6089112 | http://www.ncbi.nlm.nih.gov/pubmed/6089112 |
| PubMed | 7686882 | http://www.ncbi.nlm.nih.gov/pubmed/7686882 |
| PubMed | 7811004 | http://www.ncbi.nlm.nih.gov/pubmed/7811004 |
| PubMed | 8248233 | http://www.ncbi.nlm.nih.gov/pubmed/8248233 |
| PubMed | 8621650 | http://www.ncbi.nlm.nih.gov/pubmed/8621650 |
| PubMed | 8635474 | http://www.ncbi.nlm.nih.gov/pubmed/8635474 |
| PubMed | 8962066 | http://www.ncbi.nlm.nih.gov/pubmed/8962066 |
| PubMed | 9144789 | http://www.ncbi.nlm.nih.gov/pubmed/9144789 |
| PubMed | 9148951 | http://www.ncbi.nlm.nih.gov/pubmed/9148951 |
| PubMed | 9245398 | http://www.ncbi.nlm.nih.gov/pubmed/9245398 |
| PubMed | 9278503 | http://www.ncbi.nlm.nih.gov/pubmed/9278503 |
| PubMed | 9298644 | http://www.ncbi.nlm.nih.gov/pubmed/9298644 |
| PubMed | 9334322 | http://www.ncbi.nlm.nih.gov/pubmed/9334322 |
| PubMed | 9657678 | http://www.ncbi.nlm.nih.gov/pubmed/9657678 |
| RefSeq | WP_000072067 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=WP_000072067 |
| RefSeq | YP_026241 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=YP_026241 |
| SMART | SM00387 | http://smart.embl.de/smart/do_annotation.pl?DOMAIN=SM00387 |
| SMART | SM00433 | http://smart.embl.de/smart/do_annotation.pl?DOMAIN=SM00433 |
| SMR | P0AES6 | http://swissmodel.expasy.org/repository/smr.php?sptr_ac=P0AES6 |
| STRING | 511145.b3699 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=511145.b3699&targetmode=cogs |
| STRING | COG0187 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=COG0187&targetmode=cogs |
| SUPFAM | SSF54211 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF54211 |
| SUPFAM | SSF55874 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF55874 |
| SUPFAM | SSF56719 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF56719 |
| TIGRFAMs | TIGR01059 | http://cmr.jcvi.org/tigr-scripts/CMR/HmmReport.cgi?hmm_acc=TIGR01059 |
| UniProtKB-AC | P0AES6 | http://www.uniprot.org/uniprot/P0AES6 |
| UniProtKB | GYRB_ECOLI | http://www.uniprot.org/uniprot/GYRB_ECOLI |
| charge | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/charge/swissprot:GYRB_ECOLI |
| eggNOG | COG0187 | http://eggnogapi.embl.de/nog_data/html/tree/COG0187 |
| eggNOG | ENOG4105C7D | http://eggnogapi.embl.de/nog_data/html/tree/ENOG4105C7D |
| epestfind | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/epestfind/swissprot:GYRB_ECOLI |
| garnier | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/garnier/swissprot:GYRB_ECOLI |
| helixturnhelix | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/helixturnhelix/swissprot:GYRB_ECOLI |
| hmoment | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/hmoment/swissprot:GYRB_ECOLI |
| iep | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/iep/swissprot:GYRB_ECOLI |
| inforesidue | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/inforesidue/swissprot:GYRB_ECOLI |
| octanol | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/octanol/swissprot:GYRB_ECOLI |
| pepcoil | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepcoil/swissprot:GYRB_ECOLI |
| pepdigest | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepdigest/swissprot:GYRB_ECOLI |
| pepinfo | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepinfo/swissprot:GYRB_ECOLI |
| pepnet | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepnet/swissprot:GYRB_ECOLI |
| pepstats | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepstats/swissprot:GYRB_ECOLI |
| pepwheel | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepwheel/swissprot:GYRB_ECOLI |
| pepwindow | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/pepwindow/swissprot:GYRB_ECOLI |
| sigcleave | swissprot:GYRB_ECOLI | http://rest.g-language.org/emboss/sigcleave/swissprot:GYRB_ECOLI |
| DataBase | ID | URL or Descriptions |
|---|---|---|
| # BioGrid | 4262132 | 260 |
| # CATALYTIC ACTIVITY | GYRA_ECOLI | ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO 0000255|HAMAP-Rule MF_01897, ECO 0000269|PubMed 12051842, ECO 0000269|PubMed 18642932, ECO 0000269|PubMed 186775, ECO 0000269|PubMed 19965760, ECO 0000269|PubMed 9278055}. |
| # DOMAIN | GYRA_ECOLI | An N-terminal fragment (residues 1-523) can be reconstituted with GyrB, but the complex no longer has negative supercoiling or ATP-independent DNA relaxation activities, although it is capable of DNA cleavage; ATP-dependent relaxation is inhibited by novobiocin and non-hydrolyzable ATP analogs (PubMed 8962066). The fragment has ATP-dependent DNA relaxation and 30-fold improved decatenation activities, unlike holoenzyme it preferentially binds supercoiled DNA (PubMed 8962066). This N- terminal fragment becomes a topoisomerase IV-like enzyme; it poorly complements a temperature-sensitive parC mutation (parC is the topoisomerase IV paralog of gyrA) (PubMed 8962066). {ECO 0000269|PubMed 8962066}. |
| # DOMAIN | GYRA_ECOLI | The C-terminal domain (CTD, approximately residues 535- 841) contains 6 tandemly repeated subdomains known as blades, each of which is composed of a 4-stranded antiparallel beta-sheet (PubMed 15897198). The blades form a circular-shaped beta-pinwheel fold arranged in a spiral around a screw axis, to which DNA probably binds, inducing strong positive superhelicity (about 0.8 links/protein) (PubMed 15897198). The non-conserved, C-terminal acidic tail (residues 842-875) regulates wrapping and DNA-binding by the CTD; deletions within the tail show it is autoinhibitory for DNA wrapping and binding, and couples ATP hydrolysis to DNA strand passage (PubMed 22457353). The GyrA-box is a 7 amino acid motif found in the first blade of the CTD which is discriminative for gyrase versus topoisomerase IV activity (PubMed 9426128). The GyrA-box is required for wrapping of DNA around gyrase, and thus is essential for the DNA supercoiling activity but not DNA relaxation or decatenation activities of gyrase (PubMed 16332690). {ECO 0000269|PubMed 15897198, ECO 0000269|PubMed 16332690, ECO 0000269|PubMed 22457353, ECO 0000305|PubMed 9426128}. |
| # DrugBank | DB00537 | Ciprofloxacin |
| # ENZYME REGULATION | GYRA_ECOLI | Gyrase is the target of many classes of inhibitors, including coumarins, cyclothialidines, pyrrolopyrimidines, pyrazolthiazoles and (fluoro)quinolones. Quinolones bind GyrA when the enzyme is complexed with DNA and trap the enzyme in a covalent reaction intermediate with DNA (PubMed 3031051, PubMed 12051842). Coumarins bind to GyrB and are competitive inhibitors of its ATPase activity (PubMed 7811004). Cyclothialidines also bind GyrB and are ATPase competitive inhibitors; they seem to act differently from coumarins (PubMed 7811004). Pyrrolopyrimidines inhibit both GyrB and its paralog in topoisomerase IV (parE) (PubMed 23294697). Pyrazolthiazoles also inhibit the ATPase activity of GyrB (PubMed 20356737). DNA supercoiling and relaxation are both inhibited by oxolinic acid (PubMed 337300). Acriflavine inhibits supercoiling activity and DNA-stimulated ATPase activity (PubMed 9148951). DNA supercoiling activity is protected from fluoroquinolone inhibition by QnrB4; QnrB4 has no effect on supercoiling activity alone (PubMed 19060136). {ECO 0000269|PubMed 19060136, ECO 0000269|PubMed 20356737, ECO 0000269|PubMed 23294697, ECO 0000269|PubMed 337300, ECO 0000269|PubMed 7811004, ECO 0000269|PubMed 9148951}. |
| # EcoGene | EG10423 | gyrA |
| # FUNCTION | GYRA_ECOLI | A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to maintain chromosomes in an underwound state (PubMed 3031051, PubMed 186775, PubMed 7811004, PubMed 9148951, PubMed 12051842, PubMed 18642932, PubMed 19060136, PubMed 20356737, PubMed 22457353, PubMed 23294697, PubMed 19965760). This makes better substrates for topoisomerase IV (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli (PubMed 9334322). Gyrase catalyzes the interconversion of other topological isomers of dsDNA rings, including catenanes (PubMed 22457352). Relaxes negatively supercoiled DNA in an ATP-independent manner (PubMed 337300). E.coli gyrase has higher supercoiling activity than many other bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli (PubMed 22457352). E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical (PubMed 17400739). The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C- terminal domain (approximately residues 524-841) and specifically the GyrA-box (PubMed 8962066, PubMed 16332690). {ECO 0000269|PubMed 12051842, ECO 0000269|PubMed 16332690, ECO 0000269|PubMed 17400739, ECO 0000269|PubMed 18642932, ECO 0000269|PubMed 186775, ECO 0000269|PubMed 19060136, ECO 0000269|PubMed 19965760, ECO 0000269|PubMed 20356737, ECO 0000269|PubMed 22457352, ECO 0000269|PubMed 22457353, ECO 0000269|PubMed 23294697, ECO 0000269|PubMed 3031051, ECO 0000269|PubMed 337300, ECO 0000269|PubMed 7811004, ECO 0000269|PubMed 8962066, ECO 0000269|PubMed 9148951, ECO 0000269|PubMed 9334322}. |
| # FUNCTION | GYRA_ECOLI | Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. |
| # GO_component | GO:0005694 | chromosome; IEA:InterPro. |
| # GO_component | GO:0005737 | cytoplasm; IDA:UniProtKB. |
| # GO_component | GO:0005829 | cytosol; IDA:EcoCyc. |
| # GO_component | GO:0009295 | nucleoid; IBA:GO_Central. |
| # GO_component | GO:0009330 | DNA topoisomerase complex (ATP-hydrolyzing); IBA:GO_Central. |
| # GO_component | GO:0016020 | membrane; IDA:UniProtKB. |
| # GO_function | GO:0003677 | DNA binding; IDA:EcoliWiki. |
| # GO_function | GO:0003918 | DNA topoisomerase type II (ATP-hydrolyzing) activity; IDA:CACAO. |
| # GO_function | GO:0005524 | ATP binding; IEA:UniProtKB-HAMAP. |
| # GO_function | GO:0008094 | DNA-dependent ATPase activity; IDA:EcoliWiki. |
| # GO_function | GO:0034335 | DNA supercoiling activity; IDA:UniProtKB. |
| # GO_process | GO:0006265 | DNA topological change; IMP:EcoliWiki. |
| # GO_process | GO:0006268 | DNA unwinding involved in DNA replication; IBA:GO_Central. |
| # GO_process | GO:0006351 | transcription, DNA-templated; IDA:EcoliWiki. |
| # GO_process | GO:0007059 | chromosome segregation; IBA:GO_Central. |
| # GO_process | GO:0042493 | response to drug; IMP:EcoliWiki. |
| # GO_process | GO:0046677 | response to antibiotic; IEA:UniProtKB-KW. |
| # GOslim_component | GO:0005575 | cellular_component |
| # GOslim_component | GO:0005694 | chromosome |
| # GOslim_component | GO:0005737 | cytoplasm |
| # GOslim_component | GO:0005829 | cytosol |
| # GOslim_component | GO:0043234 | protein complex |
| # GOslim_function | GO:0003677 | DNA binding |
| # GOslim_function | GO:0016853 | isomerase activity |
| # GOslim_function | GO:0016887 | ATPase activity |
| # GOslim_function | GO:0043167 | ion binding |
| # GOslim_process | GO:0006259 | DNA metabolic process |
| # GOslim_process | GO:0007059 | chromosome segregation |
| # GOslim_process | GO:0008150 | biological_process |
| # GOslim_process | GO:0009058 | biosynthetic process |
| # GOslim_process | GO:0034641 | cellular nitrogen compound metabolic process |
| # GOslim_process | GO:0051276 | chromosome organization |
| # Gene3D | 1.10.268.10 | -; 1. |
| # Gene3D | 3.30.1360.40 | -; 1. |
| # Gene3D | 3.90.199.10 | -; 1. |
| # HAMAP | MF_01897 | GyrA |
| # INTERACTION | GYRA_ECOLI | Self; NbExp=2; IntAct=EBI-547129, EBI-547129; P0AES6 gyrB; NbExp=5; IntAct=EBI-547129, EBI-541911; P27245 marR; NbExp=2; IntAct=EBI-547129, EBI-6409744; |
| # IntAct | P0AES4 | 51 |
| # InterPro | IPR002205 | Topo_IIA_A/C |
| # InterPro | IPR005743 | GyrA |
| # InterPro | IPR006691 | GyrA/parC_pinwhl |
| # InterPro | IPR013757 | Topo_IIA_A_a |
| # InterPro | IPR013758 | Topo_IIA_A/C_ab |
| # InterPro | IPR013760 | Topo_IIA-like_dom |
| # InterPro | IPR024946 | Arg_repress_C-like |
| # KEGG_Brite | ko01000 | Enzymes |
| # KEGG_Brite | ko03032 | DNA replication proteins |
| # KEGG_Brite | ko03400 | DNA repair and recombination proteins |
| # MISCELLANEOUS | GYRA_ECOLI | Few gyrases are as efficient as E.coli at forming negative supercoils (PubMed 22457352, PubMed 17400739). Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes. {ECO 0000255|HAMAP-Rule MF_01897, ECO 0000269|PubMed 17400739, ECO 0000269|PubMed 22457352}. |
| # MISCELLANEOUS | GYRA_ECOLI | When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between GyrA and DNA (PubMed 3031051). In the presence of quinolones this intermediate can be trapped and is used as an indicator of drug toxicity (PubMed 12051842). The enzyme-DNA intermediate is also the target of a number of topoisomerase poisons, including toxin CcdB (PubMed 1324324, PubMed 8254658). {ECO 0000269|PubMed 1324324, ECO 0000269|PubMed 3031051, ECO 0000269|PubMed 8254658, ECO 0000305|PubMed 12051842}. |
| # Organism | GYRA_ECOLI | Escherichia coli (strain K12) |
| # PATRIC | 32119821 | VBIEscCol129921_2320 |
| # PDB | 1AB4 | X-ray; 2.80 A; A=30-522 |
| # PDB | 1X75 | X-ray; 2.80 A; A/B=363-494 |
| # PDB | 1ZI0 | X-ray; 2.60 A; A/B=535-841 |
| # PDB | 2Y3P | X-ray; 2.62 A; A/B=2-523 |
| # PDB | 3NUH | X-ray; 3.10 A; A=1-525 |
| # PDB | 4ELY | X-ray; 1.93 A; A/B=363-497 |
| # PIR | S02340 | ITECAP |
| # Pfam | PF00521 | DNA_topoisoIV |
| # Pfam | PF03989 | DNA_gyraseA_C; 6 |
| # Proteomes | UP000000318 | Chromosome |
| # Proteomes | UP000000625 | Chromosome |
| # RecName | DNA gyrase subunit A {ECO:0000255|HAMAP-Rule | MF_01897} |
| # RefSeq | NP_416734 | NC_000913.3 |
| # RefSeq | WP_001281242 | NZ_LN832404.1 |
| # SIMILARITY | Belongs to the type II topoisomerase GyrA/ParC subunit family. {ECO:0000255|HAMAP-Rule | MF_01897}. |
| # SMART | SM00434 | TOP4c |
| # SUBCELLULAR LOCATION | GYRA_ECOLI | Cytoplasm {ECO 0000255|HAMAP-Rule MF_01897}. |
| # SUBUNIT | GYRA_ECOLI | Heterotetramer, composed of two GyrA and two GyrB chains (PubMed 9148951, PubMed 12051842). In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with the DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis (PubMed 12051842, PubMed 18642932, PubMed 19965760, PubMed 9148951). Can form a 2 2 complex with toxin CcdB in which GyrA is inactive; rejuvenation of GyrA(2)CcdB(2) is effected by CcdA (PubMed 15854646, PubMed 1324324, PubMed 8254658, PubMed 8604132). {ECO 0000269|PubMed 12051842, ECO 0000269|PubMed 1324324, ECO 0000269|PubMed 15854646, ECO 0000269|PubMed 18642932, ECO 0000269|PubMed 19965760, ECO 0000269|PubMed 8254658, ECO 0000269|PubMed 8604132, ECO 0000269|PubMed 9148951}. |
| # SUPFAM | SSF56719 | SSF56719 |
| # eggNOG | COG0188 | LUCA |
| # eggNOG | ENOG4105C24 | Bacteria |
| BLAST | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/kblast/swissprot:GYRA_ECOLI |
| BioCyc | ECOL316407:JW2225-MONOMER | http://biocyc.org/getid?id=ECOL316407:JW2225-MONOMER |
| BioCyc | EcoCyc:EG10423-MONOMER | http://biocyc.org/getid?id=EcoCyc:EG10423-MONOMER |
| BioCyc | MetaCyc:EG10423-MONOMER | http://biocyc.org/getid?id=MetaCyc:EG10423-MONOMER |
| COG | COG0188 | http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0188 |
| DIP | DIP-36179N | http://dip.doe-mbi.ucla.edu/dip/Browse.cgi?ID=DIP-36179N |
| DOI | 10.1002/elps.1150180805 | http://dx.doi.org/10.1002/elps.1150180805 |
| DOI | 10.1006/jmbi.1993.1609 | http://dx.doi.org/10.1006/jmbi.1993.1609 |
| DOI | 10.1006/jmbi.1996.0102 | http://dx.doi.org/10.1006/jmbi.1996.0102 |
| DOI | 10.1007/BF00338386 | http://dx.doi.org/10.1007/BF00338386 |
| DOI | 10.1016/0022-2836(87)90479-7 | http://dx.doi.org/10.1016/0022-2836(87)90479-7 |
| DOI | 10.1016/0022-2836(92)90629-X | http://dx.doi.org/10.1016/0022-2836(92)90629-X |
| DOI | 10.1016/S0022-2836(02)00048-7 | http://dx.doi.org/10.1016/S0022-2836(02)00048-7 |
| DOI | 10.1016/j.bmcl.2010.03.052 | http://dx.doi.org/10.1016/j.bmcl.2010.03.052 |
| DOI | 10.1016/j.bmcl.2012.11.073 | http://dx.doi.org/10.1016/j.bmcl.2012.11.073 |
| DOI | 10.1016/j.jmb.2005.03.049 | http://dx.doi.org/10.1016/j.jmb.2005.03.049 |
| DOI | 10.1021/bi800480j | http://dx.doi.org/10.1021/bi800480j |
| DOI | 10.1038/42294 | http://dx.doi.org/10.1038/42294 |
| DOI | 10.1038/msb4100049 | http://dx.doi.org/10.1038/msb4100049 |
| DOI | 10.1046/j.1365-2958.1997.6242005.x | http://dx.doi.org/10.1046/j.1365-2958.1997.6242005.x |
| DOI | 10.1073/pnas.73.11.3872 | http://dx.doi.org/10.1073/pnas.73.11.3872 |
| DOI | 10.1073/pnas.74.11.4772 | http://dx.doi.org/10.1073/pnas.74.11.4772 |
| DOI | 10.1073/pnas.93.25.14416 | http://dx.doi.org/10.1073/pnas.93.25.14416 |
| DOI | 10.1074/jbc.272.20.13302 | http://dx.doi.org/10.1074/jbc.272.20.13302 |
| DOI | 10.1074/jbc.M112.345678 | http://dx.doi.org/10.1074/jbc.M112.345678 |
| DOI | 10.1074/jbc.M112.345736 | http://dx.doi.org/10.1074/jbc.M112.345736 |
| DOI | 10.1074/jbc.M502838200 | http://dx.doi.org/10.1074/jbc.M502838200 |
| DOI | 10.1074/jbc.M511160200 | http://dx.doi.org/10.1074/jbc.M511160200 |
| DOI | 10.1093/dnares/4.2.91 | http://dx.doi.org/10.1093/dnares/4.2.91 |
| DOI | 10.1101/gad.11.19.2580 | http://dx.doi.org/10.1101/gad.11.19.2580 |
| DOI | 10.1111/j.1365-2958.1987.tb01932.x | http://dx.doi.org/10.1111/j.1365-2958.1987.tb01932.x |
| DOI | 10.1126/science.1179123 | http://dx.doi.org/10.1126/science.1179123 |
| DOI | 10.1126/science.277.5331.1453 | http://dx.doi.org/10.1126/science.277.5331.1453 |
| DOI | 10.1128/AAC.33.6.886 | http://dx.doi.org/10.1128/AAC.33.6.886 |
| DOI | 10.1128/AAC.34.6.1271 | http://dx.doi.org/10.1128/AAC.34.6.1271 |
| DOI | 10.1128/AAC.35.2.335 | http://dx.doi.org/10.1128/AAC.35.2.335 |
| DOI | 10.1128/AAC.35.2.387 | http://dx.doi.org/10.1128/AAC.35.2.387 |
| DOI | 10.1128/AAC.38.9.1966 | http://dx.doi.org/10.1128/AAC.38.9.1966 |
| DOI | 10.1128/JB.00083-07 | http://dx.doi.org/10.1128/JB.00083-07 |
| DOI | 10.1128/JB.01205-08 | http://dx.doi.org/10.1128/JB.01205-08 |
| DrugBank | DB00537 | http://www.drugbank.ca/drugs/DB00537 |
| EC_number | EC:5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055} | http://www.genome.jp/dbget-bin/www_bget?EC:5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055} |
| EMBL | AP009048 | http://www.ebi.ac.uk/ena/data/view/AP009048 |
| EMBL | M15631 | http://www.ebi.ac.uk/ena/data/view/M15631 |
| EMBL | U00096 | http://www.ebi.ac.uk/ena/data/view/U00096 |
| EMBL | X06373 | http://www.ebi.ac.uk/ena/data/view/X06373 |
| EMBL | X06744 | http://www.ebi.ac.uk/ena/data/view/X06744 |
| EMBL | Y00544 | http://www.ebi.ac.uk/ena/data/view/Y00544 |
| ENZYME | 5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055} | http://enzyme.expasy.org/EC/5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055} |
| EchoBASE | EB0418 | http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0418 |
| EcoGene | EG10423 | http://www.ecogene.org/geneInfo.php?eg_id=EG10423 |
| EnsemblBacteria | AAC75291 | http://www.ensemblgenomes.org/id/AAC75291 |
| EnsemblBacteria | AAC75291 | http://www.ensemblgenomes.org/id/AAC75291 |
| EnsemblBacteria | BAA16048 | http://www.ensemblgenomes.org/id/BAA16048 |
| EnsemblBacteria | BAA16048 | http://www.ensemblgenomes.org/id/BAA16048 |
| EnsemblBacteria | BAA16048 | http://www.ensemblgenomes.org/id/BAA16048 |
| EnsemblBacteria | b2231 | http://www.ensemblgenomes.org/id/b2231 |
| G-Links | NC_000913 | http://link.g-language.org/NC_000913/format=tsv |
| GO_component | GO:0005694 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005694 |
| GO_component | GO:0005737 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005737 |
| GO_component | GO:0005829 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005829 |
| GO_component | GO:0009295 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009295 |
| GO_component | GO:0009330 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009330 |
| GO_component | GO:0016020 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016020 |
| GO_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GO_function | GO:0003918 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003918 |
| GO_function | GO:0005524 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005524 |
| GO_function | GO:0008094 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0008094 |
| GO_function | GO:0034335 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0034335 |
| GO_process | GO:0006265 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006265 |
| GO_process | GO:0006268 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006268 |
| GO_process | GO:0006351 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006351 |
| GO_process | GO:0007059 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0007059 |
| GO_process | GO:0042493 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0042493 |
| GO_process | GO:0046677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0046677 |
| GOslim_component | GO:0005575 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005575 |
| GOslim_component | GO:0005694 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005694 |
| GOslim_component | GO:0005737 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005737 |
| GOslim_component | GO:0005829 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005829 |
| GOslim_component | GO:0043234 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043234 |
| GOslim_function | GO:0003677 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0003677 |
| GOslim_function | GO:0016853 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016853 |
| GOslim_function | GO:0016887 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0016887 |
| GOslim_function | GO:0043167 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0043167 |
| GOslim_process | GO:0006259 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0006259 |
| GOslim_process | GO:0007059 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0007059 |
| GOslim_process | GO:0008150 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0008150 |
| GOslim_process | GO:0009058 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009058 |
| GOslim_process | GO:0034641 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0034641 |
| GOslim_process | GO:0051276 | http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0051276 |
| Gene3D | 1.10.268.10 | http://www.cathdb.info/version/latest/superfamily/1.10.268.10 |
| Gene3D | 3.30.1360.40 | http://www.cathdb.info/version/latest/superfamily/3.30.1360.40 |
| Gene3D | 3.90.199.10 | http://www.cathdb.info/version/latest/superfamily/3.90.199.10 |
| GeneID | 946614 | http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=946614 |
| HAMAP | MF_01897 | http://hamap.expasy.org/unirule/MF_01897 |
| HOGENOM | HOG000076278 | http://pbil.univ-lyon1.fr/cgi-bin/view-tree.pl?query=HOG000076278&db=HOGENOM6 |
| InParanoid | P0AES4 | http://inparanoid.sbc.su.se/cgi-bin/gene_search.cgi?id=P0AES4 |
| IntAct | P0AES4 | http://www.ebi.ac.uk/intact/pages/interactions/interactions.xhtml?query=P0AES4* |
| IntEnz | 5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055} | http://www.ebi.ac.uk/intenz/query?cmd=Search&q=5.99.1.3 {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:12051842, ECO:0000269|PubMed:18642932, ECO:0000269|PubMed:186775, ECO:0000269|PubMed:19965760, ECO:0000269|PubMed:9278055} |
| InterPro | IPR002205 | http://www.ebi.ac.uk/interpro/entry/IPR002205 |
| InterPro | IPR005743 | http://www.ebi.ac.uk/interpro/entry/IPR005743 |
| InterPro | IPR006691 | http://www.ebi.ac.uk/interpro/entry/IPR006691 |
| InterPro | IPR013757 | http://www.ebi.ac.uk/interpro/entry/IPR013757 |
| InterPro | IPR013758 | http://www.ebi.ac.uk/interpro/entry/IPR013758 |
| InterPro | IPR013760 | http://www.ebi.ac.uk/interpro/entry/IPR013760 |
| InterPro | IPR024946 | http://www.ebi.ac.uk/interpro/entry/IPR024946 |
| KEGG_Brite | ko01000 | http://www.genome.jp/dbget-bin/www_bget?ko01000 |
| KEGG_Brite | ko03032 | http://www.genome.jp/dbget-bin/www_bget?ko03032 |
| KEGG_Brite | ko03400 | http://www.genome.jp/dbget-bin/www_bget?ko03400 |
| KEGG_Gene | ecj:JW2225 | http://www.genome.jp/dbget-bin/www_bget?ecj:JW2225 |
| KEGG_Gene | eco:b2231 | http://www.genome.jp/dbget-bin/www_bget?eco:b2231 |
| KEGG_Orthology | KO:K02469 | http://www.genome.jp/dbget-bin/www_bget?KO:K02469 |
| MINT | MINT-201682 | http://mint.bio.uniroma2.it/mint/search/search.do?queryType=protein&interactorAc=MINT-201682 |
| OMA | EMKSAYI | http://omabrowser.org/cgi-bin/gateway.pl?f=DisplayGroup&p1=EMKSAYI |
| PDB | 1AB4 | http://www.ebi.ac.uk/pdbe-srv/view/entry/1AB4 |
| PDB | 1X75 | http://www.ebi.ac.uk/pdbe-srv/view/entry/1X75 |
| PDB | 1ZI0 | http://www.ebi.ac.uk/pdbe-srv/view/entry/1ZI0 |
| PDB | 2Y3P | http://www.ebi.ac.uk/pdbe-srv/view/entry/2Y3P |
| PDB | 3NUH | http://www.ebi.ac.uk/pdbe-srv/view/entry/3NUH |
| PDB | 4ELY | http://www.ebi.ac.uk/pdbe-srv/view/entry/4ELY |
| PDBsum | 1AB4 | http://www.ebi.ac.uk/pdbsum/1AB4 |
| PDBsum | 1X75 | http://www.ebi.ac.uk/pdbsum/1X75 |
| PDBsum | 1ZI0 | http://www.ebi.ac.uk/pdbsum/1ZI0 |
| PDBsum | 2Y3P | http://www.ebi.ac.uk/pdbsum/2Y3P |
| PDBsum | 3NUH | http://www.ebi.ac.uk/pdbsum/3NUH |
| PDBsum | 4ELY | http://www.ebi.ac.uk/pdbsum/4ELY |
| PSORT-B | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/kpsortb/swissprot:GYRA_ECOLI |
| PSORT2 | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/kpsort2/swissprot:GYRA_ECOLI |
| PSORT | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/kpsort/swissprot:GYRA_ECOLI |
| Pfam | PF00521 | http://pfam.xfam.org/family/PF00521 |
| Pfam | PF03989 | http://pfam.xfam.org/family/PF03989 |
| Phobius | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/kphobius/swissprot:GYRA_ECOLI |
| PhylomeDB | P0AES4 | http://phylomedb.org/?seqid=P0AES4 |
| ProteinModelPortal | P0AES4 | http://www.proteinmodelportal.org/query/uniprot/P0AES4 |
| PubMed | 12051842 | http://www.ncbi.nlm.nih.gov/pubmed/12051842 |
| PubMed | 1324324 | http://www.ncbi.nlm.nih.gov/pubmed/1324324 |
| PubMed | 15854646 | http://www.ncbi.nlm.nih.gov/pubmed/15854646 |
| PubMed | 15897198 | http://www.ncbi.nlm.nih.gov/pubmed/15897198 |
| PubMed | 16332690 | http://www.ncbi.nlm.nih.gov/pubmed/16332690 |
| PubMed | 16738553 | http://www.ncbi.nlm.nih.gov/pubmed/16738553 |
| PubMed | 17400739 | http://www.ncbi.nlm.nih.gov/pubmed/17400739 |
| PubMed | 1850970 | http://www.ncbi.nlm.nih.gov/pubmed/1850970 |
| PubMed | 1850972 | http://www.ncbi.nlm.nih.gov/pubmed/1850972 |
| PubMed | 18642932 | http://www.ncbi.nlm.nih.gov/pubmed/18642932 |
| PubMed | 186775 | http://www.ncbi.nlm.nih.gov/pubmed/186775 |
| PubMed | 19060136 | http://www.ncbi.nlm.nih.gov/pubmed/19060136 |
| PubMed | 19965760 | http://www.ncbi.nlm.nih.gov/pubmed/19965760 |
| PubMed | 20356737 | http://www.ncbi.nlm.nih.gov/pubmed/20356737 |
| PubMed | 2168148 | http://www.ncbi.nlm.nih.gov/pubmed/2168148 |
| PubMed | 22457352 | http://www.ncbi.nlm.nih.gov/pubmed/22457352 |
| PubMed | 22457353 | http://www.ncbi.nlm.nih.gov/pubmed/22457353 |
| PubMed | 23294697 | http://www.ncbi.nlm.nih.gov/pubmed/23294697 |
| PubMed | 2548439 | http://www.ncbi.nlm.nih.gov/pubmed/2548439 |
| PubMed | 2828631 | http://www.ncbi.nlm.nih.gov/pubmed/2828631 |
| PubMed | 2830458 | http://www.ncbi.nlm.nih.gov/pubmed/2830458 |
| PubMed | 2834621 | http://www.ncbi.nlm.nih.gov/pubmed/2834621 |
| PubMed | 3029031 | http://www.ncbi.nlm.nih.gov/pubmed/3029031 |
| PubMed | 3031051 | http://www.ncbi.nlm.nih.gov/pubmed/3031051 |
| PubMed | 337300 | http://www.ncbi.nlm.nih.gov/pubmed/337300 |
| PubMed | 7811004 | http://www.ncbi.nlm.nih.gov/pubmed/7811004 |
| PubMed | 8254658 | http://www.ncbi.nlm.nih.gov/pubmed/8254658 |
| PubMed | 8604132 | http://www.ncbi.nlm.nih.gov/pubmed/8604132 |
| PubMed | 8962066 | http://www.ncbi.nlm.nih.gov/pubmed/8962066 |
| PubMed | 9148951 | http://www.ncbi.nlm.nih.gov/pubmed/9148951 |
| PubMed | 9205837 | http://www.ncbi.nlm.nih.gov/pubmed/9205837 |
| PubMed | 9278055 | http://www.ncbi.nlm.nih.gov/pubmed/9278055 |
| PubMed | 9278503 | http://www.ncbi.nlm.nih.gov/pubmed/9278503 |
| PubMed | 9298644 | http://www.ncbi.nlm.nih.gov/pubmed/9298644 |
| PubMed | 9334322 | http://www.ncbi.nlm.nih.gov/pubmed/9334322 |
| PubMed | 9426128 | http://www.ncbi.nlm.nih.gov/pubmed/9426128 |
| RefSeq | NP_416734 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=NP_416734 |
| RefSeq | WP_001281242 | http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=WP_001281242 |
| SMART | SM00434 | http://smart.embl.de/smart/do_annotation.pl?DOMAIN=SM00434 |
| SMR | P0AES4 | http://swissmodel.expasy.org/repository/smr.php?sptr_ac=P0AES4 |
| STRING | 511145.b2231 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=511145.b2231&targetmode=cogs |
| STRING | COG0188 | http://string-db.org/newstring_cgi/show_network_section.pl?identifier=COG0188&targetmode=cogs |
| SUPFAM | SSF56719 | http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?ipid=SSF56719 |
| SWISS-2DPAGE | P0AES4 | http://world-2dpage.expasy.org/swiss-2dpage/protein/ac=P0AES4 |
| UniProtKB-AC | P0AES4 | http://www.uniprot.org/uniprot/P0AES4 |
| UniProtKB | GYRA_ECOLI | http://www.uniprot.org/uniprot/GYRA_ECOLI |
| charge | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/charge/swissprot:GYRA_ECOLI |
| eggNOG | COG0188 | http://eggnogapi.embl.de/nog_data/html/tree/COG0188 |
| eggNOG | ENOG4105C24 | http://eggnogapi.embl.de/nog_data/html/tree/ENOG4105C24 |
| epestfind | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/epestfind/swissprot:GYRA_ECOLI |
| garnier | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/garnier/swissprot:GYRA_ECOLI |
| helixturnhelix | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/helixturnhelix/swissprot:GYRA_ECOLI |
| hmoment | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/hmoment/swissprot:GYRA_ECOLI |
| iep | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/iep/swissprot:GYRA_ECOLI |
| inforesidue | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/inforesidue/swissprot:GYRA_ECOLI |
| octanol | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/octanol/swissprot:GYRA_ECOLI |
| pepcoil | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepcoil/swissprot:GYRA_ECOLI |
| pepdigest | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepdigest/swissprot:GYRA_ECOLI |
| pepinfo | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepinfo/swissprot:GYRA_ECOLI |
| pepnet | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepnet/swissprot:GYRA_ECOLI |
| pepstats | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepstats/swissprot:GYRA_ECOLI |
| pepwheel | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepwheel/swissprot:GYRA_ECOLI |
| pepwindow | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/pepwindow/swissprot:GYRA_ECOLI |
| sigcleave | swissprot:GYRA_ECOLI | http://rest.g-language.org/emboss/sigcleave/swissprot:GYRA_ECOLI |